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020 _a9781071634370
_9978-1-0716-3437-0
024 7 _a10.1007/978-1-0716-3437-0
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aTissue-Resident Macrophages
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Elvira Mass.
250 _a1st ed. 2024.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2024.
300 _aXVI, 576 p. 128 illus., 120 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2713
505 0 _aMacrophage Development and Function -- Fate Mapping Macrophages: From Ontogeny to Functions -- Studying Autophagy in Microglia: Overcoming the Obstacles -- Hemocyte Nuclei Isolation from Adult Drosophila melanogaster for snRNA-Seq -- Isolation of Tissue Macrophages in Adult Zebrafish -- Genetic and Immunohistochemistry Tools to Visualize Rat Macrophages In Situ -- Phenotyping of Macrophages in Human Immune System Mice -- Fate-Mapping of Yolk Sac-Derived Macrophages -- Fate-Mapping of Hematopoietic Stem Cell-Derived Macrophages -- Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue -- Isolation and Flow Cytometry Analysis of Macrophages from the Dermis -- Isolation and Flow Cytometry Analysis of Macrophages from the Kidney -- Isolation and Flow Cytometry Analysis of Intestinal Macrophages -- Isolation and Characterization of Testis Macrophages Using Flow Cytometry -- Studying Macrophages in the Murine Fatty Liver Using Flow Cytometry and Confocal Microscopy -- Isolation, Ex Vivo Expansion, and Lentiviral Transduction of Alveolar Macrophages -- Translatome Profiling of Tissue-Resident Macrophages Using the RiboTag Approach -- Spectral Flow Cytometry Analysis of Resident Tissue Macrophages -- Unveiling Macrophage Heterogeneity and Their Spatial Distribution Using Multiplexed Tissue Imaging -- 3D Imaging of Macrophages in Complete Organs -- Whole-Mount Imaging of Adipose Tissue Macrophages -- Functional In Vivo Imaging of Macrophages -- Elucidating Immune Monitoring of Tissue-Resident Macrophages by Intravital Microscopy -- Combined Host-Pathogen Fate Mapping to Investigate Lung Macrophages in Viral Infection -- Measuring the Metabolic State of Tissue-Resident Macrophages via SCENITH -- Analyzing Fcg-Receptor Interactions on Monocytes with the Proximity Ligation Assay (PLA) -- Studying Efferocytosis Dynamics in Tissue-Resident Macrophages Ex Vivo -- Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 1: Cell Preparationand Inflammasome Stimulation -- Monitoring of Inflammasome Activation of Macrophages and Microglia In Vitro, Part 2: Assessing Inflammasome Activation -- Detection of G-Quadruplex DNA Structures in Macrophages -- Adaptation of Human iPSC-Derived Macrophages Towards an Alveolar Macrophage-Like Phenotype Post Intra-Pulmonary Transfer into Murine Models -- Tackling Tissue Macrophage Heterogeneity by SplitCre Transgenesis -- Automated Cell Counting of Macrophages In Situ -- Morphometric Analyses of Macrophages -- Combined Analysis of mRNA Expression and Open Chromatin in Microglia.
520 _aThis detailed book delves into the diverse techniques and applications to target, isolate, image, phenotype, and analyze tissue-resident and monocyte-derived macrophages. The contents aim to describe the current knowledge about macrophage development and function which forces the scientific field to move beyond the previously described M1/M2 macrophage paradigm to be able to dissect macrophage functions within their specific niches during health and disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Tissue-Resident Macrophages: Methods and Protocols provides scientists entering the macrophage field with information and tools that allow them to dive into the state-of-the-art methodology used in this vital field.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aCytology.
650 0 _aImmunology.
650 0 _aBacteria.
650 1 4 _aCell Biology.
650 2 4 _aImmunology.
650 2 4 _aBacteria.
700 1 _aMass, Elvira.
_eeditor.
_0(orcid)0000-0003-2318-2356
_1https://orcid.org/0000-0003-2318-2356
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071634363
776 0 8 _iPrinted edition:
_z9781071634387
776 0 8 _iPrinted edition:
_z9781071634394
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2713
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3437-0
912 _aZDB-2-PRO
999 _c6467
_d6467