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020 _a9781071633588
_9978-1-0716-3358-8
024 7 _a10.1007/978-1-0716-3358-8
_2doi
050 4 _aTP248.13-248.65
072 7 _aTCB
_2bicssc
072 7 _aSCI010000
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072 7 _aTCB
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082 0 4 _a660.6
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245 1 0 _aPCR
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Lucília Domingues.
250 _a2nd ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXII, 254 p. 47 illus., 30 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2967
505 0 _aDigital PCR: A partitioning-based application for detection and surveillance of SARS-CoV-2 from sewage samples -- Digital PCR: A tool to authenticate herbal products and spice -- Emulsion Polymerase Chain Reaction coupled with Denaturant Gradient Gel Electrophoresis for microbial diversity studies -- Real-Time PCR High-Resolution Melting Assays for the Detection of Foodborne Pathogens -- High-Throughput real-time qPCR and High-Resolution Melting (HRM) assay for fungal detection in plant matrices -- Multiplex real-time PCR for the detection of Shiga Toxin-producing Escherichia coli in foods -- DNA isolation from cocoa-derived products and cocoa authentication by TaqMan real-time PCR -- Quantitative real-time PCR for the detection of allergenic species in foods -- Accurate absolute quantification of bacterial populations in mixed cultures by qPCR -- Real-time PCR method for assessment of ParA-mediated recombination efficiency in minicircle production -- Gene expression quantification from pathogenic bacterial biofilms by quantitative PCR -- A real-time quantitative PCR protocol for the quantification of plasmid copy number in Lactococcus lactis -- Improved PCR by the use of disruptors, a new class of oligonucleotide reagents -- Mitochondrial DNA D-loop amplification and sequencing for species differentiation in milk -- Long-range Polymerase Chain Reaction -- Megaprimer-based PCR to synthesise fusion genes for cloning -- Bacteria and Yeast ColonyPCR -- Inverse PCR for site-directed mutagenesis -- Optimized design of degenerate primers for PCR based on DNA or protein sequence comparisons. .
520 _aThis second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBiotechnology.
650 1 4 _aBiotechnology.
700 1 _aDomingues, Lucília.
_eeditor.
_0(orcid)0000-0003-1089-7627
_1https://orcid.org/0000-0003-1089-7627
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071633571
776 0 8 _iPrinted edition:
_z9781071633595
776 0 8 _iPrinted edition:
_z9781071633601
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2967
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3358-8
912 _aZDB-2-PRO
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