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020 _a9781071633236
_9978-1-0716-3323-6
024 7 _a10.1007/978-1-0716-3323-6
_2doi
050 4 _aTP248.13-248.65
050 4 _aRS189-190
072 7 _aTCB
_2bicssc
072 7 _aSCI010000
_2bisacsh
072 7 _aTCB
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082 0 4 _a660.6
_223
245 1 0 _aSingle-Cell Assays
_h[electronic resource] :
_bMicrofluidics, Genomics, and Drug Discovery /
_cedited by Paul C.H. Li, Angela Ruohao Wu.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXVI, 222 p. 64 illus., 60 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2689
505 0 _aDetection of Single Non-Small Cell Lung Cancer Cell Multi-Drug Resistance with Single Cell Bioanalyzer -- Cytosolic Calcium Measurement Utilizing a Single Cell Biochip to Study the Effect of Curcumin and Resveratrol on a Single Glioma Cell -- Dielectrophoresis-Assisted Self-Digitization Chip for High-Efficiency Single-Cell Analysis -- Optical pH Monitoring in Microdroplet Platforms for Live Cell Experiments Using Colloidal Surfactants -- PicoShells: Hollow Hydrogel Microparticles for High-Throughput Screening of Clonal Libraries -- Single Cell Micropatterning by Non-Fouling Hydrogels -- Microfluidics-Enabled Isolation and Single-Cell Analysis of Circulating Tumor Cells -- Discrimination of Multidrug Resistance in Cancer Cells Achieved Using Single-Cell Analysis -- Microfluidic Approach for Modelling Coupled Circadian Clock -- A High-Throughput Single-Cell Assay on a Valve-Based Microfluidic Platform Applied to Protein Quantification, Immune Response Monitoring, and Drug Discovery -- Single-Cell Exogenous Gene Transfection Analysis Chip -- Double Emulsion Flow Cytometry for Rapid Single Genome Detection -- Single-Cell Analysis of circRNA Using ddPCR -- A SINC-Seq Protocol for the Analysis of Subcellular Gene Expression in Single Cells -- Profiling Single-Cell Genome and Transcriptome by scONE-Seq -- Single Extracellular Vesicle Analysis Using Droplet Microfluidics.
520 _aThis detailed volume explores the use of single-cell assays in research for drug discovery, microfluidics, and more. The book delves into methodologies involving a variety of cell types and diseases, small molecules and biologics, as well as studies of the genome and transciptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Single-Cell Assays: Microfluidics, Genomics, and Drug Discovery serves to enable researchers to obtain a rapid overview in state-of-the-art microfluidic single-cell assays and an impression of what possibilities these assays offer to drug discovery.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBiological assay.
650 0 _aMicrofluidics.
650 0 _aDrug delivery systems.
650 0 _aPharmacology.
650 1 4 _aAssay Systems.
650 2 4 _aMicrofluidics.
650 2 4 _aDrug Delivery.
650 2 4 _aPharmacology.
700 1 _aLi, Paul C.H.
_eeditor.
_0(orcid)0000-0002-7946-542X
_1https://orcid.org/0000-0002-7946-542X
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aWu, Angela Ruohao.
_eeditor.
_0(orcid)0000-0002-3531-4830
_1https://orcid.org/0000-0002-3531-4830
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071633229
776 0 8 _iPrinted edition:
_z9781071633243
776 0 8 _iPrinted edition:
_z9781071633250
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2689
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3323-6
912 _aZDB-2-PRO
999 _c6463
_d6463