000 05044nam a22006255i 4500
001 978-1-0716-3275-8
003 DE-He213
005 20260610083647.0
007 cr nn 008mamaa
008 230710s2023 xxu| s |||| 0|eng d
020 _a9781071632758
_9978-1-0716-3275-8
024 7 _a10.1007/978-1-0716-3275-8
_2doi
050 4 _aQH324
072 7 _aPS
_2bicssc
072 7 _aPDN
_2bicssc
072 7 _aSCI008000
_2bisacsh
072 7 _aPS
_2thema
072 7 _aPDN
_2thema
082 0 4 _a570.28
_223
245 1 0 _aSchmidtea Mediterranea
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Luca Gentile.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXI, 280 p. 54 illus., 50 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2680
505 0 _aBuilding Phylogenies from Transcriptomic Data -- Isolation and Library Preparation of Planarian piRNAs -- Genome-Wide Analysis of Planarian piRNAs -- Combining Fluorescent In Situ Hybridization with Immunofluorescence and Lectin Staining in Planarians -- Colorimetric Whole-Mount In Situ Hybridization in Planarians -- Single Molecule Fluorescent In Situ Hybridization (smFISH) on Whole-Mount Planarians -- Whole-Mount In Situ Hybridization in Large Sexual Schmidtea mediterranea -- Preparing Planarian Cells for High-Content Fluorescence Microscopy Using RNA In Situ Hybridization and Immunocytochemistry -- An RNA/DNA-Based Flow Cytometry Approach for Isolating Slow-Cycling Stem Cells -- ACME Dissociation-Fixation, Flow Cytometry, and Cell Sorting of Freshwater Planarian Cells -- Papain-Based Dissociation of Schmidtea mediterranea Cells -- Live Immunostaining and Flow Cytometry of Schmidtea mediterranea Cells -- Live Imaging in Planarians: Immobilization and Real-Time Visualization of Reactive Oxygen Species -- A Planarian Model System to Study Host-Pathogen Interactions -- TUNEL Staining in Sections of Paraffin-Enabled Planarians -- Quantitative Analysis of Planarian Pigmentation -- mRNA Transfection of S. mediterranea Cells for Luminescence Analysis.
520 _aThis detailed volume examines fine-tuned methodologies using the planarian species, Schmidtea mediterranea. The book features experimental protocols covering topics from in situ hybridization, immunohistochemistry, cell dissociation and flow cytometry, to pipelines for the analysis of large datasets, as in genomics and transcriptomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Schmidtea mediterranea: Methods and Protocols provides both experts in the field and newcomers with the best possible toolbox for their everyday lab work utilizing this valuable model.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBiology
_xTechnique.
650 0 _aCytology.
650 1 4 _aExperimental Organisms.
650 2 4 _aCell Biology.
700 1 _aGentile, Luca.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071632741
776 0 8 _iPrinted edition:
_z9781071632765
776 0 8 _iPrinted edition:
_z9781071632772
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2680
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3275-8
912 _aZDB-2-PRO
999 _c6462
_d6462