000 06178nam a22006375i 4500
001 978-1-0716-3179-9
003 DE-He213
005 20260610083647.0
007 cr nn 008mamaa
008 230710s2023 xxu| s |||| 0|eng d
020 _a9781071631799
_9978-1-0716-3179-9
024 7 _a10.1007/978-1-0716-3179-9
_2doi
050 4 _aRC902-918
072 7 _aMJR
_2bicssc
072 7 _aMED055000
_2bisacsh
072 7 _aMJR
_2thema
082 0 4 _a616.61
_223
245 1 0 _aKidney Research
_h[electronic resource] :
_bExperimental Protocols /
_cedited by Tim D. Hewitson, Nigel D. Toussaint, Edward R. Smith.
250 _a3rd ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXV, 353 p. 87 illus., 76 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2664
505 0 _aRestoration of podocyte phenotype in culture -- Protocols for the propagation, culture and characterization of kidney fibroblasts -- Isolation of rat glomeruli and propagation of mesangial cells to study the kidney in health and disease -- In vitro models to evaluate molecular permeability of the kidney filtration barrier -- Generating human glomeruli from pluripotent stem cells for disease modelling and drug screening -- Large-scale production of kidney organoids from human pluripotent stem cells -- Construction of a multitubular perfusable kidney-on-chip for the study of renal disease -- A “kidney-on-the-chip” model composed of primary human tubular, endothelial, and white blood cells -- Standardized protocol for the preparation of precision-cut kidney slices: a translational model of renal fibrosis -- Culture of three-dimensional Madin-Darby Canine Kidney (MDCK) cysts for in vitro drug testing in polycystic kidney disease -- Zebrafishmodel to study podocyte function within the glomerular filtration barrier -- Magnetic Resonance Imaging (MRI) analysis of tissue sodium concentration in chronic kidney disease -- Determination of interstitial collagen deposition and related topography using the second harmonic generation-based Histoindex platform -- Quantitative imaging of podocyte foot processes in the kidney using confocal and STED microscopy -- Synthesis and expression of a targeted, ferritin-based tracer for PET imaging of kidney glomeruli -- Multiplex in situ hybridization in the study of acute kidney injury -- Spatial transcriptomics in kidney tissue -- Assessment of kidney mitochondrial function by high-resolution respirometry, transmission electron microscopy and histological techniques -- Transdermal measurement of glomerular filtration rate in preclinical research -- The BioHybrid Assay – a novel method for determining calcification propensity -- Quantification of calciprotein particles (CPPs) in serum/plasma samples using a fluorescent bisphosphonate -- Monitoring of Rho GTPase Activity in Podocytes.
520 _aThis third edition provides updated and new chapters on recent innovations in basic renal research. Chapters are divided into three sections covering in vitro, in vivo, and ex vivo models of kidney disease, recent advances in imaging techniques and protocols for performing analytical and functional measurements in the kidney; techniques that are both topical and of widespread relevance to the study of kidney biology and disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Kidney Research: Experimental Protocols, Third Edition aims to be comprehensive guide for researchers in the field.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aNephrology.
650 0 _aMedicine
_xResearch.
650 0 _aBiology
_xResearch.
650 1 4 _aNephrology.
650 2 4 _aBiomedical Research.
700 1 _aHewitson, Tim D.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aToussaint, Nigel D.
_eeditor.
_0(orcid)0000-0002-2853-5096
_1https://orcid.org/0000-0002-2853-5096
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aSmith, Edward R.
_eeditor.
_0(orcid)0000-0002-2651-1787
_1https://orcid.org/0000-0002-2651-1787
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071631782
776 0 8 _iPrinted edition:
_z9781071631805
776 0 8 _iPrinted edition:
_z9781071631812
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2664
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3179-9
912 _aZDB-2-PRO
999 _c6461
_d6461