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020 _a9781071632796
_9978-1-0716-3279-6
024 7 _a10.1007/978-1-0716-3279-6
_2doi
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050 4 _aQR184.5
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072 7 _aPDN
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082 0 4 _a571.96028
_223
245 1 0 _aGenotype Phenotype Coupling
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Stefan Zielonka, Simon Krah.
250 _a2nd ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXV, 408 p. 75 illus., 65 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2681
505 0 _aQuantitative Determination of Staphylococcus aureus Using Aptamer-Based Recognition and DNA Amplification Machinery -- Construction of Synthetic VHH Libraries in Ribosome Display Format -- Isolation of Adhirons Specific for Plant Protoplast Membrane Biomarkers is Simplified by Phagemid Design -- Facile One-Step Generation of Camelid VHH and Avian scFv Libraries for Phage Display by Golden Gate Cloning -- Identification of New Antibodies Targeting Tumor Cell Surface Antigens by Phage Display -- Phage Display of Bovine Ultralong CDRH3 -- Bacterial Cell Display for Selection of Affibody Molecules -- Isolation of Antigen-Specific Unconventional Bovine Ultra-Long CDR3H Antibodies Using Cattle Immunization in Combination with Yeast Surface Display -- Selection of High-Affinity Heterodimeric Antigen-Binding Fc Fragments from a Large Yeast Display Library -- A Two-Step Golden Gate Cloning Procedure for the Generation of Natively Paired YSD Fab Libraries -- Single-Cell B-Cell Sequencing to Generate Natively-Paired scFab Yeast Surface Display Libraries -- One-Pot Droplet RT-OE-PCR for the Generation of Natively Paired Antibody Immune Libraries -- Affinity Maturation of the Natural Ligand (B7-H6) for Natural Cytotoxicity Receptor NKp30 by Yeast Surface Display -- Accessing Transient Binding Pockets by Protein Engineering and Yeast Surface Display Screening -- Tyrosine Phosphorylation Screening on the Yeast Surface by Magnetic Bead Selection and FACS -- Bulk Reformatting of Antibody Fragments Displayed on the Surface of Yeast Cells to Final IgG Format for Mammalian Production -- Antibody Secreting Cell Isolation from Different Species for Microfluidic Antibody Hit Discovery -- Efficient Microfluidics Downstream Processes for Rapid Antibody Hit Confirmation -- Cell Line Development Using Targeted Gene Integration into MAR-Rich Landing Pads for Stable Expression of Transgenes -- Generation of Human 293-F Suspension NGFR Knockout Cells Using CRISPR/Cas9 Coupled to Fluorescent Protein Expression -- Antibody Display Technology (ADbody) to Present Challenging and Unstable Target Proteins on Antibodies -- SUMO: In Silico Sequence Assessment Using Multiple Optimization Parameters -- Streamlined Data Analysis Pipeline for Deep Sequence-Coupled Biopanning Identification of Pathogen-Specific Antibody Responses in Serum.
520 _aThis detailed new edition broadens the scope of the first edition by moving beyond classical display technologies. This book explores methodologies for the generation of natively paired antibody libraries, single cell technologies, alternative scaffolds, and in silico antibody sequence assessments are described. The application of these methods may allow for a generation of improved therapeutics and diagnostic reagents in a shorter time frame. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genotype Phenotype Coupling: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to expand their knowledge of antibody-based therapeutics.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBiology
_xTechnique.
650 0 _aExperimental immunology.
650 0 _aGenetics.
650 0 _aBiologicals.
650 0 _aBiopharmaceutics.
650 1 4 _aImmunological Techniques.
650 2 4 _aGenotype.
650 2 4 _aBiologics.
700 1 _aZielonka, Stefan.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aKrah, Simon.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071632789
776 0 8 _iPrinted edition:
_z9781071632802
776 0 8 _iPrinted edition:
_z9781071632819
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2681
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3279-6
912 _aZDB-2-PRO
999 _c6460
_d6460