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020 _a9781071633380
_9978-1-0716-3338-0
024 7 _a10.1007/978-1-0716-3338-0
_2doi
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072 7 _aPSF
_2bicssc
072 7 _aSCI017000
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082 0 4 _a571.6
_223
245 1 0 _aPhagocytosis and Phagosomes
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Roberto J. Botelho.
250 _a2nd ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXI, 387 p. 75 illus., 53 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2692
505 0 _aBacterial Binding, Phagocytosis, and Killing: Measurements Using Colony Forming Units -- Analysis of Human and Mouse Neutrophil Phagocytosis by Flow Cytometry -- Quantifying Phagocytosis by Immunofluorescence and Microscopy -- Methods for Quantitative Efferocytosis Assays -- Analysis of Efferocytic Receptor Dynamics and Synapse Formation in a Frustrated Efferocytosis Model -- Super-Resolution Spinning-Disk Confocal Microscopy Using Optical Photon Reassignment (SoRa) to Visualize the Actin Cytoskeleton in Macrophages -- Filamentous Bacteria as Targets to Study Phagocytosis -- The Derivation and Use of HoxB8-Driven Conditionally Immortalized Macrophages -- Quantitative Immunofluorescence to Study Phagosome Maturation and Resolution -- Approaches to Measuring Reductive and Oxidative Events in Phagosomes -- Measuring Phagosomal pH by Fluorescence Microscopy -- Multiplexed Phagosomal Assays for the Detection and Quantification of Bidirectional Exchange Between the Phagolysosomal Lumen and Extracellular Space -- Quantitative Spatio-Temporal Analysis of Phagosome Maturation in Live Cells -- Measurement of Salmonella enterica Internalization and Vacuole Lysis in Epithelial Cells -- Microscopy-Based Tracking and Quantification Methods to Study Phagosome Resolution -- Isolation of Polystyrene Bead-Induced Phagosomes for Western Blotting -- Biochemically Reconstituted Fusion of Phagosomes with Endosomes and Lysosomes -- An In Vitro System to Analyze Generation and Degradation of Phagosomal Phosphatidylinositol Phosphates -- Examining Phagosomal Pattern Recognition Receptor-Dependent Signaling and Antigen MHC-II Presentation from Phagosomes in Dendritic Cells -- Examining the Kinetics of Phagocytosis-Coupled Inflammasome Activation in Murine Bone Marrow-Derived Dendritic Cells -- Analysis of LC3-Associated Phagocytosis and Antigen Presentation in Macrophages and B Cells -- Visualizing Phagocytic Cargo In Vivo from Engulfment to Resolution in Caenorhabditis elegans -- Assessing the Phagosome Proteome by Quantitative Mass Spectrometry -- Using Ion Substitution and Fluid Indicators to Monitor Macropinosome Dynamics in Live Cells.
520 _aThis volume explores updated and entirely new experimental approaches used to investigate phagocytosis and phagosome maturation. In order to aid in the study of engulfment, maturation, resolution, and pathogen manipulation of phagocytes, the book features methodology to quantify uptake and maturation specific to certain phagocytes, particles, or pathogens, while other chapters offer methods that can be applied generically across the field. Methods are presented to study phagocytosis and phagosome maturation in vivo, in cellulo, and through in vitro analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Phagocytosis and Phagosomes: Methods and Protocols, Second Edition serves as a vital resource for both experts in the field as well as for investigators delving into phagocytosis and phagosome maturation for the first time.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aCytology.
650 0 _aCytology
_xTechnique.
650 0 _aMicrobiology.
650 1 4 _aCell Biology.
650 2 4 _aCytological Techniques.
650 2 4 _aMicrobiology.
700 1 _aBotelho, Roberto J.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071633373
776 0 8 _iPrinted edition:
_z9781071633397
776 0 8 _iPrinted edition:
_z9781071633403
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2692
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3338-0
912 _aZDB-2-PRO
999 _c6458
_d6458