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020 _a9781071633311
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024 7 _a10.1007/978-1-0716-3331-1
_2doi
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072 7 _aMED062000
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082 0 4 _a571.978
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245 1 0 _aInflammation and Cancer
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Brendan J. Jenkins.
250 _a2nd ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXVI, 373 p. 72 illus., 61 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2691
505 0 _aIdentifying Adult Stomach Tissue Stem/Progenitor Cells using the Iqgap3-2A-CreERT2 Mouse -- In Vitro and In Vivo Models for Metastatic Intestinal Tumors using Genotype-Defined Organoids -- On Target: An Intrapulmonary Transplantation Method for Modelling Lung Tumor Development in Its Native Microenvironment -- Endoscopic Ultrasound-Guided Fine Needle Biopsies to Generate Preclinical Disease Models to Study Inflammation in Pancreatic Ductal Adenocarcinoma -- Modeling Intestinal Carcinogenesis using In Vitro Organoid Cultures -- An In Vitro Model for Assessing Acute Lung Injury during Pancreatitis Development using Primary Mouse Cell Co-Cultures -- Tracking the Host Response to Infection in Peritoneal Models of Acute Resolving Inflammation -- Assessing Lung Inflammation and Pathology in Preclinical Models of Chronic Obstructive Pulmonary Disease -- Preclinical Mouse Model of Silicosis -- Tracking Cardiovascular Comorbidity in Models of Chronic Inflammatory Disease -- Live Imaging of Pyroptosis in Primary Murine Macrophages -- A Streamlined Method for Detecting Inflammasome-Induced ASC Oligomerization using Chemical Crosslinking -- DNA Methylation Analysis -- Flow Cytometry Identification of Cell Compartments in the Murine Brain -- Expression and Purification of Inflammasome Sensor Nod-Like Receptor Protein-1 using the Baculovirus-Insect Cell Expression System -- Dissecting lnterleukin-6 Classic- and Trans-Signaling in Inflammation and Cancer -- Selecting Therapeutic Antisense Oligonucleotides with Gene Targeting and TLR8 Potentiating Bifunctionality -- Exploring Allosteric Inhibitors of Protein Tyrosine Phosphatases through High-Throughput Screening -- Intravital Imaging of Regulatory T Cells in Inflamed Skin -- Confocal Endomicroscopy Monitoring of Tumor Formation -- Detection of Free Bioactive IL-18 and IL-18BP in Inflammatory Disorders -- MAC-seq: Coupling Low-Cost, High-Throughput RNA-seq with Image-Based Phenotypic Screening in 2D and 3D Cell Models -- Primary Intestinal Fibroblasts: Isolation, Cultivation, and Maintenance -- Lipid Nanoparticle-Mediated Delivery of miRNA Mimics to Myeloid Cells -- Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation.
520 _aThis second edition volume expands on the previous edition with an update on the broad spectrum of research models, techniques, and protocols used in laboratories by basic and clinical researchers. The chapters in this book are divided into two parts. Part One discusses the latest findings on the development and characterization of representative research models for chronic immune-based diseases and inflammation-associated cancers. Part Two covers biochemical, molecular, and cellular biological techniques that are commonly used to dissect the molecular mechanisms and cellular processes that drive the pathogenesis of certain disease states. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Inflammation and Cancer: Methods and Protocols, Second Edition is a valuable resource for those with a diverse range of laboratory-based experience, ranging from novice undergraduate students to established basic or clinical researchers who wish to diversify their existing portfolio of practical knowledge in the field. .
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aCancer.
650 1 4 _aCancer Biology.
700 1 _aJenkins, Brendan J.
_eeditor.
_0(orcid)0000-0002-7552-4656
_1https://orcid.org/0000-0002-7552-4656
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
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776 0 8 _iPrinted edition:
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776 0 8 _iPrinted edition:
_z9781071633335
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
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856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3331-1
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