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020 _a9781071632550
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024 7 _a10.1007/978-1-0716-3255-0
_2doi
050 4 _aR850.A1-854
050 4 _aQH315-320
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_2bicssc
072 7 _aMED106000
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072 7 _aMBGR
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082 0 4 _a610.72
_223
245 1 0 _aDiabetic Retinopathy
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Guei-Sheung Liu, Jiang-Hui Wang.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXII, 220 p. 46 illus., 39 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2678
505 0 _aInduction of Type 2 Diabetes in Mice to Understand Vascular Changes that Drive Diabetic Retinopathy -- Akimba Proliferative Diabetic Retinopathy Model: Understanding Molecular Mechanism and Drug Screening for the Progression of Diabetic Retinopathy -- Oxygen-Induced Retinopathy in the Rat: An Animal Model to Study the Proliferative Retinal Vascular Pathology -- Retinal Assessment Using In Vivo Electroretinography and Optical Coherence Tomography in Rodent Models of Diabetes -- A Guide to Genome Wide Association Study Design for Diabetic Retinopathy -- Single Cell RNA Sequencing (scRNA-Seq) Data Analysis of Retinal Homeostasis and Degeneration of Microglia -- Bioinformatics Tools for Bulk Gene Expression Deconvolution in Diabetic Retinopathy -- MicroRNA Profiling from Tears as a Potential Non-Invasive Method for Early Detection of Diabetes Retinopathy -- Proximity Extension Assay (PEA) Platform to Detect Vitreous Biomarkers of Diabetic Retinopathy -- Methods for Analyzing the Gut Microbiota in Diabetic Retinopathy -- Mass Spectrometry-Based Vitreous Proteomics: Validated Methods and Analysis Pipeline -- Methods for Ex Vivo AAV Transduction in Rodent and Human Retinal Explants -- Methods for RPE Sheet Construction Using iPS-CM in Conjunction with Natural Scaffold of Corneal Lenticule Derived from SMILE -- Chromatin Immunoprecipitation Assay in Mouse Retinal Tissue -- Frozen Block Tissue Staining for Eye Structure of Zebrafish Embryo -- Investigating the Function of MicroRNAs in Human Retinal Microvascular Endothelial Cells of Diabetic Retinopathy -- Genome Editing Inhibits Retinal Angiogenesis in a Mouse Model of Oxygen-Induced Retinopathy.
520 _aThis detailed volume provides various cellular and experimental animal models and research methodologies to understand the complex interrelated pathophysiological mechanisms triggered by hyperglycemia that underlie the development of diabetic retinopathy. The book covers methods for understanding the pathogenesis of diabetic retinopathy, detection and screening of biomarkers in diabetic retinopathy, as well as novel technologies for diabetic retinopathy management. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Diabetic Retinopathy: Methods and Protocols serves as an invaluable resource for scientists and clinicians who are interested in the area of ophthalmology research.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aMedicine
_xResearch.
650 0 _aBiology
_xResearch.
650 0 _aEndocrine glands.
650 0 _aOphthalmology.
650 1 4 _aBiomedical Research.
650 2 4 _aEndocrine System.
650 2 4 _aOphthalmology.
700 1 _aLiu, Guei-Sheung.
_eeditor.
_0(orcid)0000-0003-3379-724X
_1https://orcid.org/0000-0003-3379-724X
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aWang, Jiang-Hui.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071632543
776 0 8 _iPrinted edition:
_z9781071632567
776 0 8 _iPrinted edition:
_z9781071632574
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2678
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3255-0
912 _aZDB-2-PRO
999 _c6456
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