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020 _a9781071622810
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024 7 _a10.1007/978-1-0716-2281-0
_2doi
050 4 _aQH588.S83
072 7 _aPSC
_2bicssc
072 7 _aSCI072000
_2bisacsh
072 7 _aPSC
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082 0 4 _a571.6
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082 0 4 _a616.02774
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245 1 0 _aEpiblast Stem Cells
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Pierre Osteil.
250 _a1st ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aXI, 303 p. 71 illus., 62 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2490
505 0 _aEstablishment of Mouse Epiblast Stem Cells -- L-Proline Supplementation Drives Self-Renewing Mouse Embryonic Stem Cells to a Partially Primed Pluripotent State: The Early Primitive Ectoderm-Like Cell -- Generation of Epiblast-Like Cells -- Identification and Visualization of Protein Expression in Whole Mouse Embryos by Immunofluorescence -- Small Interfering RNA (siRNA) Transfection in Epiblast Stem Cells -- Measuring Endocytosis and Endosomal Uptake at Single Cell Resolution -- 3D Immunofluorescent Image Colocalization Quantification in Mouse Epiblast Stem Cells -- Flow Cytometric Characterization of Pluripotent Cell Protein Markers in Naïve, Formative, and Primed Pluripotent Stem Cells -- Exploring Chromatin Accessibility in Mouse Epiblast Stem Cells with ATAC-Seq -- A Reproducible and Dynamic Workflow for Analysis and Annotation of scRNA-Seq Data -- Complete Transcriptome Analysis by 5’-End Single-Cell RNA-Seq with Random Priming -- Global Proteomic Profiling of Embryonic Stem Cells Using iTRAQ Isobaric Tags with LC-MS/MS Quantification -- Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-Like Cells -- Generation of Gastruloids from Epiblast-Like Cells -- Definitive Endoderm from EpiSC Aggregates in Matrigel -- In Vitro Differentiation of Murine Embryonic Stem Cells (ESCs) into Primordial Germ Cell-Like Cells (PGCLCs) -- Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) into Human Primordial Germ Cell-Like Cells (hPGCLCs) In Vitro -- Differentiation of EpiLCs on Micro-Patterned Substrates Generated by Micro-Contact Printing -- Grafting of Epiblast Stem Cell into the Epiblast and Whole-Embryo Imaging to Unveil Lineage Competence -- Modeling Epiblast Shape in Implanting Mammalian Embryos.
520 _aThis book aims to deliver the most up-to-date protocols in using epiblast stem cells (EpiSC) to answer critical questions on mammalian development. Beginning with a section on the spectrum of mouse primed pluripotent stem cells, the volume continues with chapters on toolkits to characterize and study primed stem cells, as well as primed stem cells to engineer gastrulation models. Written for the highly successful Method in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epiblast Stem Cells: Methods and Protocols serves as an ideal guide to overcome the reproducibility problem by gathering detailed state-of-the-art protocols to perform comprehensive analyses, enabling the reader to opt for the appropriate cell type to model the epiblast.
650 0 _aStem cells.
650 0 _aDevelopmental biology.
650 1 4 _aStem Cell Biology.
650 2 4 _aDevelopmental Biology and Stem Cells.
700 1 _aOsteil, Pierre.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071622803
776 0 8 _iPrinted edition:
_z9781071622827
776 0 8 _iPrinted edition:
_z9781071622834
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2490
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2281-0
912 _aZDB-2-PRO
999 _c6455
_d6455