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001 978-1-59259-927-1
003 DE-He213
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007 cr nn 008mamaa
008 100301s2005 xxu| s |||| 0|eng d
020 _a9781592599271
_9978-1-59259-927-1
024 7 _a10.1385/1592599273
_2doi
050 4 _aRS1-441
072 7 _aMQP
_2bicssc
072 7 _aMED072000
_2bisacsh
072 7 _aMQP
_2thema
082 0 4 _a615.1
_223
245 1 0 _aReceptor Binding Techniques
_h[electronic resource] /
_cedited by Anthony P. Davenport.
250 _a2nd ed. 2005.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2005.
300 _aX, 240 p. 71 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v306
505 0 _aReceptor Databases and Computational Websites for Ligand Binding -- Identification of Orphan G Protein-Coupled Receptor Ligands Using FLIPR® Assays -- Quantitative Analysis of Orphan G Protein-Coupled Receptor mRNAs by TaqMan® Real-Time PCR -- mRNA -- Radioligand-Binding and Molecular-Imaging Techniques for the Quantitative Analysis of Established and Emerging Orphan Receptor Systems -- Measurement of Radioligand Binding by Scintillation Proximity Assay -- Autoradiography of Enzymes, Second Messenger Systems, and Ion Channels -- Immunocytochemical Localization of Receptors Using Light and Confocal Microscopy With Application to the Phenotypic Characterization of Knock-Out Mice -- Live Cell Imaging of G Protein-Coupled Receptors -- Imaging and Characterization of Radioligands for Positron Emission Tomography Using Quantitative Phosphor Imaging Autoradiography -- Dynamic In Vivo Imaging of Receptors in Small Animals Using Positron Emission Tomography.
520 _aMaintaining the high standards set by the successful first edition, Anthony P. Davenport and a panel of hands-on researchers from the pharmaceutical industry and academia take advantage of the latest developments to provide detailed practical methods for studying receptors in silico, in vitro, and in vivo. These readily reproducible techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knock-out mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan polymerase chain reaction (PCR). These ligand binding techniques are perfect for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state of the art, Receptor Binding Techniques, Second Edition offers academic and commercial researchers in the pharmaceutical and biotechnology industries a set of proven techniques for the successful characterization of receptors and the phenotyping of transgenic animals, including knock-outs.
650 0 _aPharmacy.
650 1 4 _aPharmacy.
700 1 _aDavenport, Anthony P.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588294203
776 0 8 _iPrinted edition:
_z9781617375569
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v306
856 4 0 _uhttps://doi.org/10.1385/1592599273
912 _aZDB-2-PRO
999 _c6425
_d6425