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020 _a9781493910472
_9978-1-4939-1047-2
024 7 _a10.1007/978-1-4939-1047-2
_2doi
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072 7 _aTCB
_2bicssc
072 7 _aSCI010000
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082 0 4 _a660.6
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245 1 0 _aCardiac Tissue Engineering
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Milica Radisic, Lauren D. Black III.
250 _a1st ed. 2014.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2014.
300 _aXII, 266 p. 77 illus., 60 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1181
505 0 _aSecond Generation Codon Optimized Minicircle (Comic) For Non-Viral Reprogramming Of Human Adult Fibroblasts -- Scalable Cardiac Differentiation Of Human Pluripotent Stem Cells as Microwell-Generated, Size Controlled Three-Dimensional Aggregates -- Preparation And Characterization Of Circulating Angiogenic Cells For Tissue Engineering Applications -- Isolation And Expansion Of C-Kit Positive Cardiac Progenitor Cells By Magnetic Cell Sorting -- Synthesis Of Aliphatic Polyester Hydrogel For Cardiac Tissue Engineering -- Fabrication Of Pegylated Fibrinogen, A Versatile Injectable Hydrogel Biomaterial -- Native Cardiac Extracellular Matrix Hydrogels For Cultivation Of Human Stem Cell-Derived Cardiomyocytes -- Magnetically-Actuated Alginate Scaffold: A Novel Platform For Promoting Tissue Organization And Vascularisation -- Shrink-Induced Biomimetic Wrinkled Substrates For Functional Cardiac Cell Alignment and Culture -- Injectable ECM Scaffolds For Cardiac Repair -- Generation Of Strip-Format Fibrin-Based Engineered Heart Tissue (EHT) -- Cell Tri-Culture For Cardiac Vascularization -- Cell Sheet Technology For Cardiac Tissue Engineering -- Design And Fabrication Of Biological Wires -- Collagen-Based Engineered Heart Muscle -- Creation Of A Bioreactor For The Application Of Variable Amplitude Mechanical Stimulation Of Fibrin-Gel Based Engineered Cardiac Tissue -- Preparation Of Acellular Myocardial Scaffolds With Well-Preserved Cardiomyocyte Lacunae, And Method For Applying Mechanical And Electrical Simulation To Tissue Construct -- Patch-Clamp Technique In ESC-Derived Cardiomyocytes -- Optogenetic Control Of Cardiomyocytes -- Methods For Assessing The Electromechanical Integration Of Human Pluripotent Stem Cell-Derived Cardiomyocyte Graft -- Quantifying Electrical Interactions Between Cardiomyocytes and Other Cells In Micropatterned Cell Pairs.
520 _aCardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe.  These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering.
650 0 _aBiotechnology.
650 0 _aRegenerative medicine.
650 1 4 _aBiotechnology.
650 2 4 _aRegenerative Medicine and Tissue Engineering.
700 1 _aRadisic, Milica.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBlack III, Lauren D.
_eeditor.
_0(orcid)0000-0002-7486-5811
_1https://orcid.org/0000-0002-7486-5811
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493910465
776 0 8 _iPrinted edition:
_z9781493910489
776 0 8 _iPrinted edition:
_z9781493946136
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1181
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-1047-2
912 _aZDB-2-PRO
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