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| 001 | 978-3-642-34410-7 | ||
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| 005 | 20260610083646.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 130202s2013 gw | s |||| 0|eng d | ||
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_a9783642344107 _9978-3-642-34410-7 |
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_a10.1007/978-3-642-34410-7 _2doi |
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_aAnalyzing Microbes _h[electronic resource] : _bManual of Molecular Biology Techniques / _cedited by Dilip Kumar Arora, Surajit Das, Mesapogu Sukumar. |
| 250 | _a1st ed. 2013. | ||
| 264 | 1 |
_aBerlin, Heidelberg : _bSpringer Berlin Heidelberg : _bImprint: Springer, _c2013. |
|
| 300 |
_aXI, 352 p. 80 illus., 62 illus. in color. _bonline resource. |
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| 336 |
_atext _btxt _2rdacontent |
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| 337 |
_acomputer _bc _2rdamedia |
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| 338 |
_aonline resource _bcr _2rdacarrier |
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| 347 |
_atext file _bPDF _2rda |
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| 490 | 1 |
_aSpringer Protocols Handbooks, _x1949-2456 |
|
| 505 | 0 | _aMicrobial DNA extraction, purification and quantitation -- Fluorescent based detection, quantitation and expression of wviral gene by qRT-PCR -- Restriction enzymes and their role in microbiology -- Genetic fingerprinting techniques for molecular characterization of microbes -- Agarose gel electrophoresis and Polyacrylamide agarose gel electrophoresis: Methods and principles -- Molecular identification of microbes -- Preservation and maintenance of microbial cultures -- Microbes from extreme environment: Molecular identification procedures -- ELISA based identification and detection of microbes -- Analysis of microbial diversity and construction of metagenomic library -- Bioinformatics tools for interpretation of data used in molecular identification -- Molecular phylogenetics of microbes -- Microarray technology: Basic concept, protocols and applications -- Microarray analysis of different functional genes of microorganisms -- DNA cloning and sequencing -- Biological sequence analysis: Algorithms and statistical methods. | |
| 520 | _aThis Springer Protocols manual is a practical guide to the application of key molecular biology techniques in microbiological research. The focus is on experimental protocols, which are presented in an easy-to-follow way, as step-by-step procedures for direct use in the laboratory. Notes on how to successfully apply the procedures are included, as well as recommendations regarding materials and suppliers. In addition to the practical protocols, important background information and representative results of experiments using the described methods are presented. Researchers in all areas applying microbial systems, such as in molecular biology, genetics, pathology, and agricultural research will find this work of great value. | ||
| 650 | 0 | _aMicrobiology. | |
| 650 | 0 | _aMedical microbiology. | |
| 650 | 0 | _aBiochemistry. | |
| 650 | 1 | 4 | _aMicrobiology. |
| 650 | 2 | 4 | _aMedical Microbiology. |
| 650 | 2 | 4 | _aBiochemistry. |
| 700 | 1 |
_aArora, Dilip Kumar. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt |
|
| 700 | 1 |
_aDas, Surajit. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt |
|
| 700 | 1 |
_aSukumar, Mesapogu. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt |
|
| 710 | 2 | _aSpringerLink (Online service) | |
| 773 | 0 | _tSpringer Nature eBook | |
| 776 | 0 | 8 |
_iPrinted edition: _z9783642344091 |
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_iPrinted edition: _z9783642344114 |
| 776 | 0 | 8 |
_iPrinted edition: _z9783662509678 |
| 830 | 0 |
_aSpringer Protocols Handbooks, _x1949-2456 |
|
| 856 | 4 | 0 | _uhttps://doi.org/10.1007/978-3-642-34410-7 |
| 912 | _aZDB-2-PRO | ||
| 999 |
_c6404 _d6404 |
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