000 03667nam a22004455i 4500
001 978-1-59259-263-0
003 DE-He213
005 20260610083646.0
007 cr nn 008mamaa
008 100301s1999 xxu| s |||| 0|eng d
020 _a9781592592630
_9978-1-59259-263-0
024 7 _a10.1385/1592592635
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
_223
245 1 0 _aEicosanoid Protocols
_h[electronic resource] /
_cedited by Elias A Lianos.
250 _a1st ed. 1999.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1999.
300 _aXI, 296 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v120
505 0 _aUse of a Retroviral Vector for Expression of a Foreign Gene -- Extraction of Eicosanoids from Biological Fluids, Cells, and Tissues -- Assessment of Cyclooxygenase RNA Expression by Northern Hybridization -- Immunohistochemical Analysis of Cyclooxygenase Expression in Human Skin -- Separation of Hydroxyeicosatetraenoic Acids and Leukotrienes Originating from Kidney Tissue by C-18 Reverse-Phase High-Pressure Liquid Chromatography -- Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry -- Assessment of Cyclooxygenase Protein Expression by Western Blotting -- Assessment of Arachidonic Acid Distribution into Phospholipids of Inflammatory Cells -- Determination of Phospholipase A2s Expression in Mast Cells by Reverse-Transcriptase Polymerase Chain Reaction -- Enzyme immunoassays (EIA) of Eicosanoids -- Transcellular Regulation of Eicosanoid Biosynthesis -- Assessment of Cellular Localization of the Thromboxane A2 Receptor by Immunocytochemistry -- Epoxyeicosatrienoic Acids -- Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts -- Assessment of 5-Lipoxygenase Activity and Cellular Distribution -- Leukotrienes, Lipoxins, and Hydroxyeicosatetraenoic Acids -- Whole Blood Assays for Evaluation of Thromboxane Synthase Inhibition -- Novel Eicosanoids.
520 _aIn Eicosanoid Protocols, Elias A. Lianos and a panel of hands-on experts present cutting-edge methods for the study of eicosanoids, including prostaglandins, thromboxanes, and leukotrienes. The readily reproducible methods described hereconcentrate on studying the regulation of expression and function of enzymes, particularly cyclooxygenase (and its two isoforms), phospholipase A2, and lipoxygenases involved in the synthesis of established eicosanoids. Additional chapters are devoted to the characterization and distribution of the thromboxane A2 receptor in tissues and the biological roles of novel eicosanoids. Timely and authoritative, the methods in this book will help their users in exploring the pathobiology of inflammation. Eicosanoid Protocols offers new and established researchers powerful, state-of-the-art tools to probe the regulation and function of eicosanoids.
650 0 _aBiochemistry.
650 1 4 _aBiochemistry.
700 1 _aLianos, Elias A.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896036673
776 0 8 _iPrinted edition:
_z9781489943620
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v120
856 4 0 _uhttps://doi.org/10.1385/1592592635
912 _aZDB-2-PRO
999 _c6397
_d6397