000 04073nam a22004695i 4500
001 978-1-59259-266-1
003 DE-He213
005 20260610083645.0
007 cr nn 008mamaa
008 100301s1999 xxu| s |||| 0|eng d
020 _a9781592592661
_9978-1-59259-266-1
024 7 _a10.1385/159259266X
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
_223
245 1 0 _aFlavoprotein Protocols
_h[electronic resource] /
_cedited by Steven K. Chapman, Graeme A. Reid.
250 _a1st ed. 1999.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1999.
300 _aXII, 256 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v131
505 0 _aUV-Visible Spectroscopy as a Tool to Study Flavoproteins -- Identifying and Quantitating FAD and FMN in Simple and in Iron-Sulfur-Containing Flavoproteins -- Fluorescence Analysis of Flavoproteins -- Potentiometric Measurement of Oxidation-Reduction Potentials -- Flavoprotein Kinetics -- Application of Freeze Quenching to the Study of Rapid Reactions in Flavoproteins -- Application of Electron Spin Resonance (ESR) for Detection and Characterization of Flavoprotein Semiquinones -- Circular Dichroism Studies of Flavoproteins -- Vibrational Spectroscopy of Flavoproteins -- NMR of Flavoproteins -- Flavoprotein Resolution and Reconstitution -- Flavoenzyme Structure and Function -- Identification of Covalent Flavoproteins and Analysis of the Covalent Link -- Flavin Synthesis and Incorporation into Synthetic Peptides -- Computational Methods in Flavin Research -- Flavins and Flavoenzymes in Diagnosis and Therapy.
520 _aAs a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow—or perhaps you already have. Alternatively, you may identify a polypeptide sequence that is related to known flavin-containing proteins. This may, or may not, be your first encounter with flavoproteins. However, even if you are an old hand in the field, you may not have exploited the full range of experimental approaches applicable to the study of flavoproteins. We hope that Flavoprotein Protocols will encourage you to do so. In this volume we have sought to bring together a range of experimental methods of value to researchers with an interest in flavoproteins, whether or not these researchers have experience in this area. A broad range of techniques, from the everyday to the more specialized, is described by scientists who are experts in their fields and who have ext- sive practical experience with flavoproteins. The wide range of approaches, from wet chemistry to dry computation, has, as a consequence, demanded a range of formats. Where appropriate (particularly for analytical methods) the protocol described is laid out in easy-to-follow steps. In other cases (e. g. , the more advanced spectroscopies and computational methods) it is far more apt to describe the general approach and relevance of the methods. We hope this wide-ranging approach will sow the seeds of many future collaborations - tween laboratories and further our knowledge and understanding of how f- voproteins work.
650 0 _aBiochemistry.
650 1 4 _aBiochemistry.
700 1 _aChapman, Steven K.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aReid, Graeme A.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896037342
776 0 8 _iPrinted edition:
_z9781617371585
776 0 8 _iPrinted edition:
_z9781489941756
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v131
856 4 0 _uhttps://doi.org/10.1385/159259266X
912 _aZDB-2-PRO
999 _c6378
_d6378