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020 _a9781071633731
_9978-1-0716-3373-1
024 7 _a10.1007/978-1-0716-3373-1
_2doi
050 4 _aQH460-468
072 7 _aPSAK1
_2bicssc
072 7 _aSCI029000
_2bisacsh
072 7 _aPSAK
_2thema
082 0 4 _a572.86459
_223
245 1 0 _aBase Excision Repair Pathway
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Kishor K. Bhakat, Tapas K. Hazra.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXIII, 262 p. 47 illus., 35 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2701
505 0 _aSimultaneous Short and Long Patch Base Excision Repair (BER) Assay in Live Mammalian Cells -- In Vitro Assay to Measure APE1 Enzymatic Activity on Ribose Monophosphate Abasic Site -- Highly Sensitive Radioactivity-Based DNA 3’-Phosphatase Activity Assay for Polynucleotide Kinase 3’-Phosphatase -- Generation of Recombinant Nucleosomes Containing Site-Specific DNA Damage -- A DNA Cleavage Assay Using Synthetic Oligonucleotide Containing a Single Site-Directed Lesion for In Vitro Base Excision Repair Study -- In Vitro Reconstitutive Base Excision Repair (BER) Assay -- Detection of Oxidatively Modified Base Lesion(s) in Defined DNA Sequences by FLARE Quantitative PCR -- Isolation and Immunodetection of Enzymatic DNA-Protein Crosslinks by RADAR Assay -- Slot Blot Assay for Detection of R Loops -- Assays with Patient-Derived Organoids to Evaluate the Impact of Microbial Infection on the Base Excision Repair (BER) Enzymes -- Characterizing the Repair of DNA Double-Strand Breaks: A Review of Surrogate Plasmid-Based Reporter Methods -- Interactome Profiling of DNA Damage Response (DDR) Mediators with Immunoprecipitation-Mass Spectrometry -- Using Affinity Pulldown Assays to Study Protein-Protein Interactions of Human NEIL1 Glycosylase and the Checkpoint Protein RAD9-RAD1-HUS1 (9-1-1) Complex -- Tandem Affinity Purification and Mass-Spectrometric Analysis of FACT and Associated Proteins -- Analysis of Copy Number Variation of DNA Repair/Damage Response Genes in Tumor Tissues -- Genome-Wide Binding Analysis of DNA Repair Protein APE1 in Tumor Cells by ChIP-Seq -- Tumorsphere Formation Assay: A Cancer Stem-Like Cell Characterization in Pediatric Brain Cancer Medulloblastoma.
520 _aThis detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering procedures to detect and quantify the damaged bases, protein DNA crosslinks, and double-strand breaks, experimental procedures to identify DNA repair protein interactome by conventional tandem affinity purification followed by mass spectroscopy analysis, as well as the analysis of genome-wide binding of DNA repair proteins and copy number variations of the DNA damage response gene in tumors. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topis, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Base Excision Repair Pathway: Methods and Protocols serves as a valuable resource for novices and experts trying to examine the repair of various types of DNA lesions in vitro and in cell by the distinct set of proteins in the BER pathway.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aDNA damage.
650 0 _aDNA repair.
650 0 _aGenomics.
650 0 _aProteins.
650 1 4 _aDNA Damage and Repair.
650 2 4 _aGenomics.
650 2 4 _aProteins.
700 1 _aBhakat, Kishor K.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aHazra, Tapas K.
_eeditor.
_0(orcid)0000-0002-4587-687X
_1https://orcid.org/0000-0002-4587-687X
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071633724
776 0 8 _iPrinted edition:
_z9781071633748
776 0 8 _iPrinted edition:
_z9781071633755
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2701
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3373-1
912 _aZDB-2-PRO
999 _c6266
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