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020 _a9781071632871
_9978-1-0716-3287-1
024 7 _a10.1007/978-1-0716-3287-1
_2doi
050 4 _aRC346-429.2
072 7 _aPSAN
_2bicssc
072 7 _aSCI089000
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072 7 _aPSAN
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082 0 4 _a616.8
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245 1 0 _aStem Cell-Based Neural Model Systems for Brain Disorders
_h[electronic resource] /
_cedited by Yu-Wen Alvin Huang, ChangHui Pak.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXIII, 292 p. 1 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2683
505 0 _aGeneration of Cerebral Cortical Neurons from Human Pluripotent Stem Cells in 3D Culture -- Generation of Homogeneous Populations of Cortical Interneurons from Human Pluripotent Stem Cells -- Generation and Co-Culture of Cortical Glutamatergic and GABAergic Induced Neuronal Cells -- Transcription Factor-Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells -- Directed Differentiation of Human iPSCs into Microglia-Like Cells Using Defined Transcription Factors -- The Generation and Functional Characterization of Human Microglia-Like Cells Derived from iPS and Embryonic Stem Cells -- Modeling Cellular Crosstalk of Neuroinflammation Axis by Tri-Cultures of iPSC-Derived Human Microglia, Astrocytes, and Neurons -- Generation of Oligodendrocytes from Human Pluripotent and Embryonic Stem Cells -- Characterizing the Neuron-Glial Interactions by the Co-Cultures of Human iPSC-Derived Oligodendroglia and Neurons -- Defined Differentiation of Human Pluripotent Stem Cells to Brain Microvascular Endothelial-Like Cells for Modeling the Blood-Brain Barrier -- Modeling the Blood-Brain Barrier Using Human Induced Pluripotent Stem Cells -- A Three-Dimensional Primary Cortical Culture System Compatible with Transgenic Disease Models, Virally-Mediated Fluorescence, and Live Microscopy -- Method to Generate Dorsal Forebrain Brain Organoids from Human Pluripotent Stem Cells -- A 3D Bioengineered Neural Tissue Model Generated from Human iPSC-Derived Neural Precursor Cells -- FACS-Based Sequencing Approach to Evaluate Cell Type to Genotype Associations Using Cerebral Organoids -- Dynamic Measurement of Endosome-Lysosome Fusion in Neurons Using High-Content Imaging -- Live-Imaging Detection of Multivesicular Body-Plasma Membrane Fusion and Exosome Release in Cultured Primary Neurons -- Assays of Monitoring and Measuring Autophagic Flux for iPSC-Derived Human Neurons and Other Brain Cell Types -- Measuring Neuronal Network Activity Using Human Induced Neuronal Cells -- A Simple Ca2+-Imaging Approach of Network-Activity Analyses for Human Neurons -- Whole Cell Patch Clamp Electrophysiology in Human Neuronal Cells -- Assaying Chemical Long-Term Potentiation in Human iPSC-Derived Neuronal Networks.
520 _aThis detailed volume presents validated and well-adapted procedures involving humanized and/or stem cell-based neural model systems that have proven helpful in better understanding the essential brain functions involved in the pathogenesis of brain disorders. The book explores the generation of multiple neural cell types in 2D and 3D as well as cutting-edge techniques to assay neural function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cell-Based Neural Model Systems for Brain Disorders serves as an essential resource for researchers and students in neuroscience, stem cell biology, and related fields.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aNervous system
_xDiseases.
650 0 _aStem cells.
650 0 _aBiological models.
650 1 4 _aNeurological Disorders.
650 2 4 _aStem Cell Biology.
650 2 4 _aBiological Models.
700 1 _aHuang, Yu-Wen Alvin.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aPak, ChangHui.
_eeditor.
_0(orcid)0000-0003-4192-7665
_1https://orcid.org/0000-0003-4192-7665
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071632864
776 0 8 _iPrinted edition:
_z9781071632888
776 0 8 _iPrinted edition:
_z9781071632895
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2683
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3287-1
912 _aZDB-2-PRO
999 _c6260
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