000 04382nam a22004455i 4500
001 978-1-59259-780-2
003 DE-He213
005 20260610083642.0
007 cr nn 008mamaa
008 100301s2005 xxu| s |||| 0|eng d
020 _a9781592597802
_9978-1-59259-780-2
024 7 _a10.1385/1592597807
_2doi
050 4 _aRC254-282
072 7 _aMJCL
_2bicssc
072 7 _aMED062000
_2bisacsh
072 7 _aMJCL
_2thema
082 0 4 _a616.994
_223
245 1 0 _aPancreatic Cancer
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Gloria H. Su.
250 _a1st ed. 2005.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2005.
300 _aXII, 352 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Medicine,
_x1940-6037 ;
_v103
505 0 _aIdentification and Analysis of Precursors to Invasive Pancreatic Cancer -- Optimal Molecular Profiling of Tissue and Tissue Components -- Immunohistochemistry and In Situ Hybridization in Pancreatic Neoplasia -- Practical Methods for Tissue Microarray Construction -- Xenografting and Harvesting Human Ductal Pancreatic Adenocarcinomas for DNA Analysis -- Culture and Immortalization of Pancreatic Ductal Epithelial Cells -- DNA Methylation Analysis in Human Cancer -- Digital Single-Nucleotide Polymorphism Analysis for Allelic Imbalance -- Representational Difference Analysis as a Tool in the Search for New Tumor Suppressor Genes -- Serial Analyses of Gene Expression (SAGE) -- Oligonucleotide-Directed Microarray Gene Profiling of Pancreatic Adenocarcinoma -- Identification of Differentially Expressed Proteins in Pancreatic Cancer Using a Global Proteomic Approach -- Detection of Telomerase Activity in Patients with Pancreatic Cancer -- Serological Analysis of Expression cDNA Libraries (SEREX) -- Modeling Pancreatic Cancer in Animals to Address Specific Hypotheses -- Strategies for the Use of Site-Specific Recombinases in Genome Engineering -- Primary Explant Cultures of Adult and Embryonic Pancreas -- Zebrafish as a Model for Pancreatic Cancer Research -- Development of a Cytokine-Modified Allogeneic Whole Cell Pancreatic Cancer Vaccine -- Overview of Linkage Analysis.
520 _aIn the last decade, major progress has been made in identifying the critical oncogenes and tumor-suppressor genes that can cause pancreatic cancer. In Pancreatic Cancer: Methods and Protocols, leading clinicians and scientists from prestigious research institutes and cancer centers present in readily reproducible format the classical techniques that have contributed to these advances in pancreatic research and introduce new strategies that should produce future breakthroughs across the field of cancer biology. The authors provide a broad range of methods for the molecular, biochemical, pathological, and statistical analyses of both sporadic and familial pancreatic cancers, methods that can be applied not only to basic, but also to translational pancreatic research. Topics covered include in vitro cell cultures, in vivo mouse models, protein studies, mutation analysis, and treatment development. The protocols follow the successful Methods in Molecular Medicine™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Pancreatic Cancer: Methods and Protocols offers those interested in either basic or translational research an ambitious collection of essential yet novel methods for pancreatic cancer research, methods that can also readily be applied broadly throughout cancer research.
650 0 _aOncology.
650 1 4 _aOncology.
700 1 _aSu, Gloria H.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588291073
776 0 8 _iPrinted edition:
_z9781617373626
830 0 _aMethods in Molecular Medicine,
_x1940-6037 ;
_v103
856 4 0 _uhttps://doi.org/10.1385/1592597807
912 _aZDB-2-PRO
999 _c6204
_d6204