000 04115nam a22004455i 4500
001 978-1-59259-861-8
003 DE-He213
005 20260610083642.0
007 cr nn 008mamaa
008 100301s2005 xxu| s |||| 0|eng d
020 _a9781592598618
_9978-1-59259-861-8
024 7 _a10.1385/1592598617
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aHuman Cell Culture Protocols
_h[electronic resource] /
_cedited by Joanna Picot.
250 _a2nd ed. 2005.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2005.
300 _aXII, 360 p. 120 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Medicine,
_x1940-6037 ;
_v107
505 0 _aEstablishment and Maintenance of Normal Human Keratinocyte Cultures -- Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters -- Isolation and Culture of Human Osteoblasts -- Human Osteoclast Culture From Peripheral Blood Monocytes -- Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation -- Human Myoblasts and Muscle-Derived SP Cells -- Cell Cultures of Autopsy-Derived Fibroblasts -- Primary Culture and Differentiation of Human Adipocyte Precursor Cells -- Human Mononuclear Phagocytes in Tissue Culture -- Purification of Peripheral Blood Natural Killer Cells -- Human Fetal Brain Cell Culture -- Culturing Human Schwann Cells -- Well-Differentiated Human Airway Epithelial Cell Cultures -- Isolation and Culture of Human Alveolar Epithelial Cells -- A New Approach to Primary Culture of Human Gastric Epithelium -- Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a Noninjurious Approach -- Isolation and Culture of Human Hepatocytes -- Glomerular Epithelial and Mesangial Cell Culture and Characterization -- Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules -- Culture of Parathyroid Cells -- Long-Term Culture and Maintenance of Human Islets of Langerhans in Memphis Serum-Free Media -- Primary Culture of Human Antral Endocrine and Epithelial Cells -- Conjunctiva Organ and Cell Culture -- Establishment, Maintenance, and Transfection of In Vitro Cultures of Human Retinal Pigment Epithelium.
520 _aIn this second edition of a popular and widely acclaimed collection of laboratory methods, a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range of human cell types relevant to human disease. Each technique can be used to investigate a wide spectrum of important processes, ranging from the pathogenesis of disease, to the study of metabolic processes, to control of proliferation and differentiation. New to this edition are chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Wide-ranging and highly practical, Human Cell Culture, Second Edition, provides novice and experienced researchers alike with a detailed, step-by-step guide to successful culture human cells today.
650 0 _aCytology.
650 1 4 _aCell Biology.
700 1 _aPicot, Joanna.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588292223
776 0 8 _iPrinted edition:
_z9781617374319
830 0 _aMethods in Molecular Medicine,
_x1940-6037 ;
_v107
856 4 0 _uhttps://doi.org/10.1385/1592598617
912 _aZDB-2-PRO
999 _c6197
_d6197