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020 _a9781493964390
_9978-1-4939-6439-0
024 7 _a10.1007/978-1-4939-6439-0
_2doi
050 4 _aQD431-431.7
072 7 _aPSBC
_2bicssc
072 7 _aSCI049000
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082 0 4 _a572.6
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245 1 0 _aActivity-Based Proteomics
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Herman S. Overkleeft, Bogdan I. Florea.
250 _a1st ed. 2017.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Humana,
_c2017.
300 _aXI, 222 p. 47 illus., 18 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1491
505 0 _aActivity-Based Protein Profiling – From Chemical Novelty to Biomedical Stalwart -- A Quantitative Mass-Spectrometry Platform to Monitor Changes in Cysteine Reactivity -- Activity-Based Protein Profiling with Natural Product-Derived Chemical Probes in Human Cell Lysates -- Inhibitor Discovery by Convolution Activity-Based Protein Profiling -- Activity-Based Protein Profiling in Bacteria -- Activity-Based Lipid Esterase Profiling of M. Bovis BCG at Different Metabolic States Using Tetrahydrolipstatin (THL) as Bait -- Comparative Activity-Based Flavin-Dependent Oxidase Profiling -- Activity-Dependent Photoaffinity Labelling of Metalloproteases -- Profiling the Activity of Deubiquitinating Enzymes using Chemically Synthesized Ubiquitin-Based Probes -- Profiling Protein Sumoylation using Biochemical and Mass Spectrometry-Based Research Tools -- Live Cell Imaging and Profiling of Cysteine Cathepsin Activity using a Quenched Activity-Based Probe -- Competitive Activity-Based Protein Profiling Of Serine Hydrolases: A Case Study on DAGL-Alpha -- A Protocol for Protein Profiling using Chemoselective Cleavable Linker Probes in Semi-Permeabilized Cells -- Cleavable Linkers in Chemical Proteomics Applications -- Two-Step Activity-Based Protein Profiling With the Proteasome System as Model of Study.
520 _aThis volume focuses on explorative activity-based proteomics, biomedical applications of activity-based proteomics, and chemical strategies in activity-based proteomics providing a concise overview of activity-based protein profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Activity-Based Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.
650 0 _aProteins .
650 1 4 _aProtein Biochemistry.
700 1 _aOverkleeft, Herman S.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aFlorea, Bogdan I.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781493964376
776 0 8 _iPrinted edition:
_z9781493964383
776 0 8 _iPrinted edition:
_z9781493982004
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1491
856 4 0 _uhttps://doi.org/10.1007/978-1-4939-6439-0
912 _aZDB-2-PRO
999 _c6185
_d6185