000 05425nam a22005535i 4500
001 978-1-59745-289-2
003 DE-He213
005 20260610083642.0
007 cr nn 008mamaa
008 130824s2009 xxu| s |||| 0|eng d
020 _a9781597452892
_9978-1-59745-289-2
024 7 _a10.1007/978-1-59745-289-2
_2doi
050 4 _aQK710-899
072 7 _aPSTD
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPSTB
_2thema
082 0 4 _a571.2
_223
245 1 0 _aPlant Signal Transduction
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Thomas Pfannschmidt.
250 _a1st ed. 2009.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2009.
300 _aXI, 360 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v479
505 0 _aMultiple Dimensions in Plant Signal Transduction: An Overview -- Phenotyping of Arabidopsis Mutants for Developmental Effects of Gene Deletions -- Phenotyping of Abiotic Responses and Hormone Treatments in Arabidopsis -- Accurate Real-time Reverse Transcription Quantitative PCR -- Probing Spatio-temporal Intracellular Calcium Variations in Plants -- Dynamic Redox Measurements with Redox-Sensitive GFP in Plants by Confocal Laser Scanning Microscopy -- Imaging of Reactive Oxygen Species In vivo -- Identification of Thioredoxin Targeted Proteins Using Thioredoxin Single-Cysteine Mutant-Immobilized Resin -- Determination of in vivo Protein Phosphorylation in Photosynthetic Membranes -- Examining Protein Stability and Its Relevance for Plant Growth and Development -- Probing Circadian Rhythms in Chlamydomonas reinhardtii by Functional Proteomics -- Bimolecular Fluorescence Complementation (BiFC) to Study Protein-protein Interactions in Living Plant Cells -- Fluorescence Cross-Correlation Spectroscopy of Plant Proteins -- The Determination of Protein-protein Interactions by the Mating-based Split-ubiquitin system (mbSUS) -- Functional Complementation of Yeast Mutants to Study Plant Signalling Pathways -- Phosphatase Activities Analyzed by in vivo Expressions -- Chromatin Immunoprecipitation Experiments to Investigate In Vivo Binding of Arabidopsis Transcription Factors to Target Sequences -- Fluorescence-based Electrophoretic Mobility Shift Assay in the Analysis of DNA-binding Proteins -- Analysis of Plant Regulatory DNA sequences by the Yeast-One-Hybrid Assay -- Analysis of Plant Regulatory DNA Sequences by Transient Protoplast Assays and Computer Aided Sequence Evaluation -- RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System.
520 _aDuring recent years, research has greatly expanded our understanding of the sophisticated molecular network of responses which enable plants to develop, survive and propagate under a wide range of conditions. In Plant Signal Transduction: Methods and Protocols, an international panel of experts provide well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in planta analyses and the proteins involved in signal transduction in order to aid with the establishment of laboratory techniques or the modification of the protocols for other plants. As part of the highly successful Methods in Molecular Biology™ series, the chapters include brief introductions to the subject, lists of necessary materials, readily reproducible laboratory protocols, and tips on trouble-shooting and avoiding known pitfalls. Comprehensive and cutting-edge, Plant Signal Transduction: Methods and Protocols will benefit plant scientists wishing to improve their experimental approaches and delve further into this exciting and important field of study.
532 8 _aAccessibility summary: This PDF is not accessible. It is based on scanned pages and does not support features such as screen reader compatibility or described non-text content (images, graphs etc). However, it likely supports searchable and selectable text based on OCR (Optical Character Recognition). Users with accessibility needs may not be able to use this content effectively. Please contact us at accessibilitysupport@springernature.com if you require assistance or an alternative format.
532 8 _aInaccessible, or known limited accessibility
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aPlant physiology.
650 0 _aBotany.
650 0 _aBotanical chemistry.
650 1 4 _aPlant Physiology.
650 2 4 _aPlant Science.
650 2 4 _aPlant Biochemistry.
700 1 _aPfannschmidt, Thomas.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781588299437
776 0 8 _iPrinted edition:
_z9781607610694
776 0 8 _iPrinted edition:
_z9781627038027
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v479
856 4 0 _uhttps://doi.org/10.1007/978-1-59745-289-2
912 _aZDB-2-PRO
999 _c6178
_d6178