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| 001 | 978-1-0716-3405-9 | ||
| 003 | DE-He213 | ||
| 005 | 20260610083642.0 | ||
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| 008 | 230802s2023 xxu| s |||| 0|eng d | ||
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_a9781071634059 _9978-1-0716-3405-9 |
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_a10.1007/978-1-0716-3405-9 _2doi |
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_a572.6 _223 |
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_aBiophysical Characterization of Functional Peptides _h[electronic resource] / _cedited by Vibin Ramakrishnan. |
| 250 | _a1st ed. 2023. | ||
| 264 | 1 |
_aNew York, NY : _bSpringer US : _bImprint: Humana, _c2023. |
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_aXIV, 139 p. 46 illus., 25 illus. in color. _bonline resource. |
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_atext _btxt _2rdacontent |
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_acomputer _bc _2rdamedia |
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_aonline resource _bcr _2rdacarrier |
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_bTable of contents navigation _2onix |
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_bSingle logical reading order _2onix |
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_bShort alternative textual descriptions _2onix |
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_bUse of color is not sole means of conveying information _2onix |
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_bUse of high contrast between text and background color _2onix |
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_bNext / Previous structural navigation _2onix |
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_bAll non-decorative content supports reading without sight _2onix |
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_atext file _bPDF _2rda |
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_aSpringer Protocols Handbooks, _x1949-2456 |
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| 505 | 0 | _aDedication -- Preface -- Acknowledgement -- Chapter 1 De novo Peptide Design Tools.-Chapter 2 Molecular Docking -- Chapter 3 Molecular Dynamics Simulations -- Chapter 4 Peptide Synthesis -- Chapter 5 Mass Spectrometry -- Chapter 6 Circular Dichroism (CD) Spectroscopy -- Chapter 7 Fourier Transform Infrared (FTIR) Spectroscopy -- Chapter 8 Cell Culture for Cell Penetrating Peptides -- Chapter 9 Comparative Uptake of Peptides by Flow Cytometry -- Chapter 10 Cellular uptake of peptides by confocal microscopy -- Chapter 11 Penetration in 3D Tumorospheres -- Chapter 12 TMRM Cytotoxicity Assay -- Chapter 13 Cell Death by Apoptosis -- Chapter 14 Hemolytic activity -- Chapter 15 Antimicrobial Assay -- Chapter 16 Antimicrobial assay with Peptide Based Hydrogels -- Chapter 17 Rheology of Peptide based Hydrogels -- Chapter 18 Estimation of Aggregation Kinetics -- Chapter 19 Assessment of Amyloid morphology using Electron Microscopy -- Chapter 20 Catalytic Efficiency of the Designed Peptides. | |
| 520 | _aThis protocol book presents reproducible and step-by-step procedures for the peptide synthesis, their characterization and applications. The volume includes an introductory section on in silico modelling of new peptide molecules, Molecular Dynamics Simulations, Docking, Electrostatic fingerprinting of peptides, and other modelling tools for peptide designing and optimization. Further, it covers protocols for the solid phase peptide synthesis, chromatographic and mass spectrometric characterization of peptides. Importantly, it covers methods for biophysical characterizations of peptides for their potential applications as drug delivery vehicles, peptide nano-assembly, bionanocatalysis, protein aggregation diseases, and peptide-based anti-bacterial. | ||
| 532 | 8 | _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com. | |
| 532 | 8 | _aNo reading system accessibility options actively disabled | |
| 532 | 8 | _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com | |
| 650 | 0 | _aProteins . | |
| 650 | 0 | _aBiophysics. | |
| 650 | 0 | _aDrug delivery systems. | |
| 650 | 1 | 4 | _aProtein Biochemistry. |
| 650 | 2 | 4 | _aBiophysics. |
| 650 | 2 | 4 | _aDrug Delivery. |
| 700 | 1 |
_aRamakrishnan, Vibin. _eeditor. _0(orcid)0000-0002-8048-3211 _1https://orcid.org/0000-0002-8048-3211 _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 710 | 2 | _aSpringerLink (Online service) | |
| 773 | 0 | _tSpringer Nature eBook | |
| 776 | 0 | 8 |
_iPrinted edition: _z9781071634042 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781071634066 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781071634073 |
| 830 | 0 |
_aSpringer Protocols Handbooks, _x1949-2456 |
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| 856 | 4 | 0 | _uhttps://doi.org/10.1007/978-1-0716-3405-9 |
| 912 | _aZDB-2-PRO | ||
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_c6171 _d6171 |
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