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020 _a9781071629505
_9978-1-0716-2950-5
024 7 _a10.1007/978-1-0716-2950-5
_2doi
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050 4 _aQP620-625
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245 1 0 _aClinical Applications of Nucleic Acid Amplification
_h[electronic resource] /
_cedited by Meagan B. Myers, Cynthia A. Schandl.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXIV, 345 p. 84 illus., 67 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2621
505 0 _aA Brief Practical Guide to PCR -- Managing a PCR Contamination Event in a Molecular Pathology Laboratory -- Next Generation Sequencing Informatic Architecture Considerations -- Nuclease Enrichment and qPCR Detection of Rare Nucleotide Variants -- MYD88 L265P Mutation Detection by ddPCR: Recommendations for Screening and Minimal Residual Disease Monitoring -- Capture Methylation-Sensitive Restriction Enzyme Sequencing (Capture MRE-Seq) for Methylation Analysis of Highly Degraded DNA -- LT-RPA: An Isothermal DNA Amplification Approach for Improved Microsatellite Genotyping and Microsatellite Instability Detection -- Liquid Biopsy for Advanced Cancer: An Amplicon Based Massively Parallel Sequencing Panel Approach to Precision Oncology -- Mate Pair Sequencing: Next Generation Sequencing for Structural Variant Detection -- Cell Subset-Specific Chimerism Testing by Short Tandem Repeats Analysis for Engraftment Monitoring After Hematopoietic Stem Cell Transplantation -- Immune Cell Lineage-Specific Chimerism Testing by Next-Generation Sequencing for Engraftment Monitoring After Allogeneic Hematopoietic Stem Cell Transplantation -- Rapid Whole Genome Sequencing for Diagnosis of Single Locus Genetic Diseases in Critically Ill Children -- EOSAL-CNV for Easy and Rapid Detection of CNVs by Fragment Analysis -- Rapid Cycle and Extreme Polymerase Chain Reaction -- CRISPR-Cas12-Based Diagnostic Applications in Infectious and Zoonotic Diseases -- SARS-CoV-2 Sequencing for Variant Surveillance -- Tuberculosis Diagnosis Using Isothermal Nucleic Acid Amplification in a Paper-and-Plastic Device -- Loop-Mediated Isothermal Amplification on Paper Microfluidic Chips for Highly Sensitive and Specific Zika Virus Detection Using Smartphone -- Ultrasensitive Reverse Transcriptase Loop-Mediated Isothermal Amplification (US-LAMP) Based Detection of Malaria Infection from Dried Blood Spots. .
520 _aThis volume details applications, methods, and protocols on nucleic acid amplification. Chapters detail the management of organ and stem cell transplantation, constitutional genetics and genomics, epidemiology, public health, nucleic acid detection, methods for amplification and sequencing, nuclease-assisted mutant allele enrichment, isothermal, and high-throughput sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Clinical Applications of Nucleic Acid Amplification is a valuable resource for both novice and expert scientists in this developing field.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBiomaterials.
650 0 _aNucleic acids.
650 0 _aBiochemistry.
650 1 4 _aNucleic Acid.
650 2 4 _aBiochemistry.
650 2 4 _aChemical Biology.
700 1 _aMyers, Meagan B.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aSchandl, Cynthia A.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071629499
776 0 8 _iPrinted edition:
_z9781071629512
776 0 8 _iPrinted edition:
_z9781071629529
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2621
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2950-5
912 _aZDB-2-PRO
999 _c6168
_d6168