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020 _a9781071621769
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024 7 _a10.1007/978-1-0716-2176-9
_2doi
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072 7 _aPNF
_2bicssc
072 7 _aSCI013010
_2bisacsh
072 7 _aPNF
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082 0 4 _a543
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245 1 0 _aAffinity Chromatography
_h[electronic resource] :
_bMethods and Protocols /
_cedited by B. Vijayalakshmi Ayyar, Sushrut Arora.
250 _a1st ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aX, 278 p. 52 illus., 42 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2466
505 0 _aAntibody Purification Using Affinity Chromatography -- TIM4-affinity Methods Targeting Phosphatidylserine for Isolation or Detection of Extracellular Vesicles -- Application of Affitins for Affinity Purification of Proteins -- Identification of Clusterin as a major ABri- and ADan-binding protein using affinity Chromatography -- Application of a Novel CL7/Im7 Affinity System in Purification of Complex and Pharmaceutical Proteins -- Positive and Negative Affinity Chromatography to Purify Norovirus P-Domain Protein -- Robotic Affinity Purification of Soluble and Insoluble Recombinant Glutathione-S-Transferase Fusion Proteins -- Application of Immunoaffinity-Mass Spectrometry (IA-MS) for Protein Biomarker Quantification -- Cellular Membrane Affinity Chromatography for the Identification of Bioactive Molecules from Complex Natural Mixtures -- Rapid Affinity Purification of Tagged Plant Mitochondria (Mito-AP) -- Arginine-affinity Chromatographyfor Nucleic Acids (DNA and RNA) Isolation -- Purification and Analysis of Nucleotides and Nucleosides from Plants -- Ligand Selection for Affinity Chromatography Using Phage Display -- Selection and Application of Aptamer Affinity for Protein Purification -- Entrapment of Proteins within Columns for High-Performance Affinity Chromatography -- Preparation of Affinity Chromatography Monolith in Miniaturized Format and Application for Protein Separation -- Ligand Immobilization Methods for Affinity Chromatography -- Preparation of Molecularly Imprinted poly(N-isopropylacrylamide) Thermosensitive Based Cryogels -- Preparation of Staphylococcal Protein a Imprinted Supermacroporous Cryogel Beads.
520 _aThis volume covers various aspects of affinity chromatography along with examples of its applications. Chapters guide readers through methodologies to purify a diverse array of molecular targets such as antibodies, extracellular vesicles, recombinant proteins, biomarkers, metabolites, plant organelles, nucleic acids, ligand identification and protocols on building affinity matrix. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Affinity Chromatography: Methods and Protocols aims to be an invaluable resource to anyone employing affinity chromatography-based methodologies.
650 0 _aAnalytical chemistry.
650 0 _aAnalytical biochemistry.
650 1 4 _aAnalytical Chemistry.
650 2 4 _aAnalytical Biochemistry.
700 1 _aAyyar, B. Vijayalakshmi.
_eeditor.
_0(orcid)0000-0001-6775-9546
_1https://orcid.org/0000-0001-6775-9546
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_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aArora, Sushrut.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071621752
776 0 8 _iPrinted edition:
_z9781071621776
776 0 8 _iPrinted edition:
_z9781071621783
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2466
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2176-9
912 _aZDB-2-PRO
999 _c6155
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