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020 _a9781071621813
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024 7 _a10.1007/978-1-0716-2181-3
_2doi
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082 0 4 _a570.28
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245 1 0 _aC. elegans
_h[electronic resource] :
_bMethods and Applications /
_cedited by Gal Haspel, Anne C. Hart.
250 _a3rd ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aXI, 388 p. 58 illus., 47 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
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490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2468
505 0 _aStarting a C. elegans Research Laboratory: Practical Advice -- The Basics of Setting Up Successful Teaching Labs and Short-Term Projects with C. elegans -- Cryopreservation of C. elegans and Other Nematodes with Dimethyl Sulfoxide and Trehalose -- Genetic Methods for Cellular Manipulation in C. elegans -- Observing and Quantifying Fluorescent Reporters -- Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior -- Optogenetic Perturbation of Individual C. elegans Pharyngeal Neurons while Monitoring Feeding Behavior -- Antibody Staining for Nematodes with Heat-Induced Antigen Retrieval (HIAR) -- ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy -- A Fusion-PCR Method for Expressing Genetic Tools in C. elegans -- Approaches for CRISPR/Cas9 Genome Editing in C. elegans -- Targeted and Random Transposon-Assisted Single-Copy Transgene Insertionin C. elegans -- Mutation Mapping and Identification by Whole-Genome Sequencing -- Lipid Extraction and Analysis -- Isolating Caenorhabditis elegans from the Natural Habitat -- Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans -- Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser -- An Imaging System for Monitoring C. elegans Behavior and Aging -- Methods for Modulating and Measuring Neuromuscular Exertion in C. elegans -- Recording and Quantifying C. elegans Behavior -- Primer on Mathematical Modeling in C. elegans.
520 _aThis detailed new edition provides a guide for implementing a selection of novel techniques utilizing the pioneering organism, Caenorhabditis elegans, a vital discovery engine for the rules of life, from molecules and genes, through cellular physiology, metabolism and aging, neurobiology and behavior, to individuality and interaction with the environment. Beginning with two chapters offering practical advice for setting up research programs with C. elegans, either for a new research group or for a short-term project such as a teaching laboratory, the volume continues with numerous hands-on methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, C. elegans: Methods and Applications, Third Edition offers the kind of clear guidance that will serve all researchers working with these vital and dynamic organisms.
650 0 _aBiological models.
650 1 4 _aBiological Models.
700 1 _aHaspel, Gal.
_eeditor.
_0(orcid)0000-0001-6701-697X
_1https://orcid.org/0000-0001-6701-697X
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aHart, Anne C.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071621806
776 0 8 _iPrinted edition:
_z9781071621820
776 0 8 _iPrinted edition:
_z9781071621837
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2468
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2181-3
912 _aZDB-2-PRO
999 _c6150
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