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020 _a9781071621646
_9978-1-0716-2164-6
024 7 _a10.1007/978-1-0716-2164-6
_2doi
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072 7 _aPST
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPST
_2thema
082 0 4 _a580
_223
245 1 0 _aProtoplast Technology
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Kan Wang, Feng Zhang.
250 _a1st ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aXII, 263 p. 43 illus., 40 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2464
505 0 _aPhyscomitrium patens Protoplasting and Protoplast Transfection -- Protoplast Isolation and Transfection in the Single-Cell C4 Species Bienertia sinuspersici -- Protoplast for Gene Functional Analysis in Arabidopsis -- Application of Protoplast Regeneration to CRISPR/Cas9 Mutagenesis in Nicotiana tabacum -- CRISPR DNA- and RNP-Mediated Genome Editing via Nicotiana benthamiana Protoplast Transformation and Regeneration -- Protoplast Isolation and Transfection in Rice -- Protoplast Isolation and Transfection in Maize -- Isolation and Transfection of Maize Endosperm Protoplasts -- Protoplast Isolation from Undifferentiated Maize Seedling Shoot Tissue -- Protoplast Isolation and Transfection in Wheat -- Protoplast Isolation, Transformation, and Regeneration for Forage and Turf Grasses -- Efficient Protoplast Isolation and DNA Transfection for Winter Oilseed Crops, Pennycress (Thlaspi arvense) and Camelina (Camelina sativa) -- Protoplast Isolation,Transfection, and Gene Editing for Soybean (Glycine max) -- Protoplast Isolation and Transformation in Oil Palm -- Protoplast Preparation and Fluorescence-Activated Cell Sorting for the Evaluation of Targeted Mutagenesis in Plant Cells -- Characterization of Programmable Transcription Activators in the Model Monocot Setaria viridis via Protoplast Transfection -- High-Throughput Transfection and Analysis of Soybean (Glycine max) Protoplasts.
520 _aThis detailed volume collects protocols from scientists who are actively engaged in developing or using protoplast technology. The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species. It continues by exploring protoplast automation, large scale functional genomics, and synthetic biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protoplast Technology: Methods and Protocols aims to inspire the new generation of researchers to further improve their protocols and apply this technology to accelerate the field of plant genomic study.
650 0 _aBotany.
650 0 _aPlant genetics.
650 1 4 _aPlant Science.
650 2 4 _aPlant Genetics.
700 1 _aWang, Kan.
_eeditor.
_0(orcid)0000-0003-2474-5800
_1https://orcid.org/0000-0003-2474-5800
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aZhang, Feng.
_eeditor.
_0(orcid)0000-0002-3539-3655
_1https://orcid.org/0000-0002-3539-3655
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071621639
776 0 8 _iPrinted edition:
_z9781071621653
776 0 8 _iPrinted edition:
_z9781071621660
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2464
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2164-6
912 _aZDB-2-PRO
999 _c6147
_d6147