| 000 | 03532nam a22004575i 4500 | ||
|---|---|---|---|
| 001 | 978-1-0716-1166-1 | ||
| 003 | DE-He213 | ||
| 005 | 20260610083641.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 210520s2021 xxu| s |||| 0|eng d | ||
| 020 |
_a9781071611661 _9978-1-0716-1166-1 |
||
| 024 | 7 |
_a10.1007/978-1-0716-1166-1 _2doi |
|
| 050 | 4 | _aQD431-431.7 | |
| 072 | 7 |
_aPSBC _2bicssc |
|
| 072 | 7 |
_aSCI049000 _2bisacsh |
|
| 072 | 7 |
_aPSB _2thema |
|
| 082 | 0 | 4 |
_a572.6 _223 |
| 245 | 1 | 0 |
_aPDZ Mediated Interactions _h[electronic resource] : _bMethods and Protocols / _cedited by Jean-Paul Borg. |
| 250 | _a1st ed. 2021. | ||
| 264 | 1 |
_aNew York, NY : _bSpringer US : _bImprint: Humana, _c2021. |
|
| 300 |
_aXI, 292 p. 100 illus., 50 illus. in color. _bonline resource. |
||
| 336 |
_atext _btxt _2rdacontent |
||
| 337 |
_acomputer _bc _2rdamedia |
||
| 338 |
_aonline resource _bcr _2rdacarrier |
||
| 347 |
_atext file _bPDF _2rda |
||
| 490 | 1 |
_aMethods in Molecular Biology, _x1940-6029 ; _v2256 |
|
| 505 | 0 | _aIdentification of PDZ Interactions by Yeast Two-Hybrid -- Identification of PDZ Interactions by Affinity Purification and Mass Spectrometry Analysis -- Identification of PDZ Interactions by Proteomic Peptide Phage Display -- A Computational Protocol to Analyze PDZ/PBM Affinity Data Obtained by High-Throughput Holdup Assay -- Study of PDZ-Peptide and PDZ-Lipid Interactions by Surface Plasmon Resonance/BIAcore -- PDZ Sample Quality Assessment by Biochemical and Biophysical Characterizations -- Crystallographic Studies of PDZ Domain-Peptide Interactions of the Scribble Polarity Module -- A Fluorescence-Based Assay to Determine PDZ-Ligand Binding Thermodynamics -- Unveiling the Folding Mechanism of PDZ Domains -- Development of Peptide-Based PDZ Domain Inhibitors -- Dynamic Control of Signaling by Phosphorylation of PDZ Binding Motifs -- Chemical Synthesis of PDZ Domains -- Viral PDZ Binding Motifs Influence Cell Behavior through the Interaction with Cellular Proteins Containing PDZ Domains -- Computational Design of PDZ:peptide Binding -- Mechanoregulation of PDZ Proteins, An Emerging Function -- Rational Design of PDZ Domain Inhibitors: Discovery of Small Organic Compounds Targeting PDZ Domains. | |
| 520 | _aThis volume provides readers with a comprehensive look at the latest techniques used to identify and characterize PDZ-mediated interactions. Chapters cover topics such as promiscuity, multimodularity, regulation, and viral recognition by PDZ domains. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, PDZ Mediated Interactions: Methods and Protocols is a valuable resource for all researchers interested in learning more about this developing field. . | ||
| 650 | 0 | _aProteins . | |
| 650 | 1 | 4 | _aProtein Biochemistry. |
| 700 | 1 |
_aBorg, Jean-Paul. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt |
|
| 710 | 2 | _aSpringerLink (Online service) | |
| 773 | 0 | _tSpringer Nature eBook | |
| 776 | 0 | 8 |
_iPrinted edition: _z9781071611654 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781071611678 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781071611685 |
| 830 | 0 |
_aMethods in Molecular Biology, _x1940-6029 ; _v2256 |
|
| 856 | 4 | 0 | _uhttps://doi.org/10.1007/978-1-0716-1166-1 |
| 912 | _aZDB-2-PRO | ||
| 999 |
_c6120 _d6120 |
||