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020 _a9781071603819
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024 7 _a10.1007/978-1-0716-0381-9
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
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245 1 0 _aCircadian Clocks
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Steven A. Brown.
250 _a1st ed. 2021.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2021.
300 _aXXII, 342 p. 64 illus., 53 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2130
505 0 _aReal-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock -- Inductively Coupled Plasma Mass Spectrometry for Elemental Analysis in Circadian Biology -- The Assessment of Circadian Rhythms within the Immune System -- Measuring Circadian Rhythms in Human Cells -- Measuring the Effects of Circadian Rhythm-Related Manipulations on Depression-Like Behavior in Rodents: Forced Swim and Tail Suspension Tests -- Asking the Clock: How to Use Information from Questionnaires for Circadian Phenotyping -- Simple Kinetic Models in Molecular Chronobiology -- Searching Novel Clock Genes using RNAi-Based Screening -- Chromatin Immunoprecipitation (ChIP) from Mouse Liver Nuclei -- Using GRO-seq to Measure Circadian Transcription and Discover Circadian Enhancers -- Circadian Metabolomics from Breath -- A Lipidomics View of Circadian Biology -- Circadian Lipidomics: Analysis of Lipid Metabolites Around-The-Clock -- Phosphoproteome and Proteome Sample Preparation from Mouse Tissues for Circadian Analysis -- Circadian Phosphorylation of CLOCK and BMAL1 -- Fluorescence Live Imaging of Drosophila Circadian Pacemaker Neurons -- Monitoring Electrical Activity in Drosophila Circadian Output Neurons -- Light Input to the Mammalian Circadian Clock -- Acute In Vivo Multi-Electrode Recordings from the Mouse Suprachiasmatic Nucleus -- Perforated Multi-Electrode Array Recording in Hypothalamic Brain Slices -- Collection of Mouse Brain Slices for Bioluminescence Imaging of Circadian Clock Networks -- Computational Analysis of PER2::LUC Imaging Data -- Electrophysiological Approaches to Studying the Suprachiasmatic Nucleus -- Optogenetic Methods for the Study of Circadian Rhythms.
520 _aThis volume presents techniques used by researchers from all branches of biology to study daily changes at a molecular level in many physiological systems. The chapters are organized into three parts and cover topics such as measuring and modeling physiological and behavioral rhythms; genome-wide analyses in circadian biology, and imaging and manipulating brain clocks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Circadian Clocks: Methods and Protocols is a valuable tool for any researcher interested in learning more about this developing field.
650 0 _aBiochemistry.
650 0 _aBiology
_xTechnique.
650 1 4 _aBiochemistry.
650 2 4 _aExperimental Organisms.
700 1 _aBrown, Steven A.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071603802
776 0 8 _iPrinted edition:
_z9781071603826
776 0 8 _iPrinted edition:
_z9781071603833
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2130
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-0381-9
912 _aZDB-2-PRO
999 _c6105
_d6105