000 04691nam a22005655i 4500
001 978-1-0716-0954-5
003 DE-He213
005 20260610083641.0
007 cr nn 008mamaa
008 201203s2021 xxu| s |||| 0|eng d
020 _a9781071609545
_9978-1-0716-0954-5
024 7 _a10.1007/978-1-0716-0954-5
_2doi
050 4 _aQK861-899
072 7 _aPST
_2bicssc
072 7 _aPSB
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPST
_2thema
072 7 _aPSB
_2thema
082 0 4 _a572.2
_223
245 1 0 _aPlant Chemical Genomics
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Glenn R. Hicks, Chunhua Zhang.
250 _a2nd ed. 2021.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2021.
300 _aXII, 196 p. 44 illus., 30 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2213
505 0 _aChemical Genetics to Uncover Mechanisms Underlying Lipid-Mediated -- Signaling Events in Plants -- Method for Phenotypic Chemical Screening to Identify Cryptochrome Inhibitor -- Whole Seedling Based Chemical Genetic Screens in Arabidopsis -- Identification of Type iii Secretion Inhibitors for Plant Disease Management -- Investigation of Drug Efficacy by Screening Bioactive Chemical Effects on Plant Cell Subcellular Architecture -- In Vivo Chemical Genomics with Random Cyclized Peptides -- Interfering Peptides Targeting Protein-Protein Interactions in the Ethylene Plant Hormone Signaling Pathway as Tools to Delay Plant Senescence -- The Screening for Novel Inhibitors of Auxin-Induced Ca2+ Signaling -- Identification of ABA Receptor Agonists Using a Multiplexed High-Throughput Chemical Screening -- A Luciferase Reporter Assay to Identify Chemical Activators of ABA Signaling -- Identification of Novel Molecular Regulators Modulating Ethylene Biosynthesis Using EMS-Based Genetic Screening -- Investigation of Auxin Biosynthesis and Action Using Auxin Biosynthesis Inhibitors -- Target Profiling of an Anticancer Drug Curcumin by an In Situ Chemical Proteomics Approach -- Label-Free Target Identification and Confirmation Using Thermal Stability Shift Assays -- Drug Affinity Responsive Target Stability (DARTS) Assay to Detect Interaction Between a Purified Protein and a Small Molecule -- Using Differential Scanning Fluorimetry (DSF) to Detect Ligand Binding with Purified Protein -- Microscale Thermophoresis (MST) to Detect the Interaction between Purified Protein and Small Molecule.
520 _aThis second edition volume is a companion volume to the previous edition and looks at new findings on novel bioactive chemicals and cognate targets, as well as the use of synthetic small molecules and a variety of tools to understand these processes. Chapters in this book cover topics such as screening plants for novel bioactive chemicals including lipid signaling and photo receptors; small molecule screens to include peptide ligands to generate new variations; using new chemicals to affect and dissect hormone signaling; and the application of easier microscale methods to simplify target identification and validation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Plant Chemical Genomics: Methods and Protocols, Second Edition is a valuable resource that provides a foundation of techniques for novice and expert researchers in the plant chemical biology community.
650 0 _aBotanical chemistry.
650 0 _aPlant genetics.
650 0 _aBotany.
650 0 _aGenetics.
650 1 4 _aPlant Biochemistry.
650 2 4 _aPlant Genetics.
650 2 4 _aPlant Science.
650 2 4 _aGenetics and Genomics.
700 1 _aHicks, Glenn R.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aZhang, Chunhua.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071609538
776 0 8 _iPrinted edition:
_z9781071609552
776 0 8 _iPrinted edition:
_z9781071609569
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2213
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-0954-5
912 _aZDB-2-PRO
999 _c6104
_d6104