000 04145nam a22005055i 4500
001 978-1-0716-0806-7
003 DE-He213
005 20260610083640.0
007 cr nn 008mamaa
008 200912s2021 xxu| s |||| 0|eng d
020 _a9781071608067
_9978-1-0716-0806-7
024 7 _a10.1007/978-1-0716-0806-7
_2doi
050 4 _aQD431-431.7
072 7 _aPSBC
_2bicssc
072 7 _aSCI049000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572.6
_223
245 1 0 _aNanopore Technology
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Monifa A.V. Fahie.
250 _a1st ed. 2021.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2021.
300 _aX, 231 p. 57 illus., 43 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2186
505 0 _aPreparation of Fragaceatoxin C (FraC) Nanopores -- Preparation of Cytolysin A (ClyA) Nanopores -- Building Synthetic Transmembrane Peptide Pores -- Design and Assembly of Membrane-Spanning DNA Nanopores -- Determining the Orientation of Porins in Planar Lipid Bilayers -- Revelation of Function and Inhibition of Wza through Single Channel Studies -- Protein Analyte Sensing with an Outer Membrane Protein G (OmpG) Nanopore -- Nanopore Enzymology to Study Protein Kinases and their Inhibition by Small Molecules -- A Selective Activity-Based Approach for Analysis of Enzymes with an OmpG Nanopore -- Oligonucleotide-Directed Protein Threading through a Rigid Nanopore -- Unfolding and Translocation of Proteins through an Alpha-Hemolysin Nanopore by ClpXP -- Simulation of pH-Dependent, Loop-Based Membrane Protein Gating Using Pretzel -- Free Energy Minimization for Vesicle Translocation through a Narrow Pore -- Single Ion-Channel Analysis in Droplet Interface Bilayer -- Continuous and Rapid Solution Exchangein a Lipid Bilayer Perfusion System Based on Droplet-Interface Bilayer -- Protein Transport Studied by a Model Asymmetric Membrane Army Arranged in a Dimple Chip.
520 _aThis detailed collection explores techniques involved in the main strategies of nanopore sensing, such as translocation, analyte trapping, and interactions with external binding sites. Opening with a section on nanopore design and nanopore production, the book continues with parts devoted to various biological nanopores, nanopore engineering, and their uses in single molecule sensing, computational methods to study intrinsic nanopore behavior, characterizing the specific translocation activity of a vesicle particle through a nanopore, as well as the use of the technique droplet interface bilayer (DIB) in nanopore and membrane biophysical studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nanopore Technology: Methods and Protocols, with its focus on nanopore technology and biomolecule characterization, will hold the interest of the biophysicists, biochemists, bioengineers, and molecular biologists who are working toward further understanding this key field of research.
650 0 _aProteins .
650 0 _aBiophysics.
650 0 _aBiotechnology.
650 1 4 _aProtein Biochemistry.
650 2 4 _aBiophysics.
650 2 4 _aBiotechnology.
700 1 _aFahie, Monifa A.V.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071608050
776 0 8 _iPrinted edition:
_z9781071608074
776 0 8 _iPrinted edition:
_z9781071608081
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2186
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-0806-7
912 _aZDB-2-PRO
999 _c6102
_d6102