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020 _a9781071607510
_9978-1-0716-0751-0
024 7 _a10.1007/978-1-0716-0751-0
_2doi
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072 7 _aPST
_2bicssc
072 7 _aSCI011000
_2bisacsh
072 7 _aPST
_2thema
082 0 4 _a580
_223
245 1 0 _aVirus-Induced Gene Silencing in Plants
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Vincent Courdavault, Sébastien Besseau.
250 _a1st ed. 2020.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2020.
300 _aXII, 261 p. 58 illus., 48 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2172
505 0 _airus-induced Gene Silencing in Lilies using Cucumber Mosaic Virus Vectors -- Virus-Induced Gene Silencing in Poaceae Using a Foxtail Mosaic Virus Vector -- Virus-Induced Gene Silencing (VIGS) in Chili Pepper (Capsicum spp.) -- Virus-Induced Gene Silencing in Diploid and Tetraploid Potato Species -- Virus Induced Gene Silencing (VIGS) in Cassava using Geminivirus Agroclones -- Virus Induced Gene Silencing of Cell Wall Genes in Flax (Linum usitatissimum) -- Virus-Induced Gene Silencing to Investigate Alkaloid Biosynthesis in Opium Poppy -- A Biolistic-mediated Virus-Induced Gene Silencing in Apocynaceae to Map Biosynthetic Pathways of Alkaloids -- Virus Induced Gene Silencing in Nepeta -- Virus Induced Gene Silencing in Sweet basil (Ocimum basilicum) -- Virus-Induced Gene Silencing for Gene Function Studies in Withania somnifera, an Important Indian Medicinal Plant -- A Prunus necrotic ringspot virus(PNRSV)-based Viral Vector for Characterization of Gene Functions in Prunus Fruit Trees -- Virus-Induced Gene Silencing in Olive Tree (Oleaceae) -- ALSV-based Virus-Induced Gene Silencing in Apple Tree (Malus x domestica L.) -- Virus-Induced Gene Silencing for Functional Analysis of Flower Traits in Petunia -- Virus-induced Gene Silencing in Rose Flowers -- Virus-induced Gene Silencing (VIGS) in Flax (Linum usitatissimum L.) Seedcoat: Description of an Effective Procedure using the transparent testa 2 Gene as a Selectable Marker -- Virus-Based microRNA Silencing in Plants.
520 _aThis volume aims at providing a complete and updated overview of gene downregulation in plants performed through virus-induced gene silencing (VIGS). Chapters guide readers through classical and newly developed protocols of VIGS to allow readers to initiate or optimize their own silencing experiments according to the methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Virus-Induced Gene Silencing in Plants: Methods and Protocols aims to ensure successful results in the further study of this vital field.
650 0 _aBotany.
650 0 _aPlant genetics.
650 1 4 _aPlant Science.
650 2 4 _aPlant Genetics.
700 1 _aCourdavault, Vincent.
_eeditor.
_0(orcid)0000-0001-8902-4532
_1https://orcid.org/0000-0001-8902-4532
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBesseau, Sébastien.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071607503
776 0 8 _iPrinted edition:
_z9781071607527
776 0 8 _iPrinted edition:
_z9781071607534
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2172
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-0751-0
912 _aZDB-2-PRO
999 _c6100
_d6100