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020 _a9781071604670
_9978-1-0716-0467-0
024 7 _a10.1007/978-1-0716-0467-0
_2doi
050 4 _aRC1-1245
072 7 _aMJC
_2bicssc
072 7 _aMED022000
_2bisacsh
072 7 _aMJC
_2thema
082 0 4 _a616
_223
245 1 0 _aGroup A Streptococcus
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Thomas Proft, Jacelyn M. S. Loh.
250 _a1st ed. 2020.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2020.
300 _aXIV, 403 p. 54 illus., 39 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2136
505 0 _aDetection of Streptococcus pyogenes Virulence Factors -- Closed-Tube Multiplex Real-Time PCR for the Detection of Group A Streptococcal Superantigens -- The emm-cluster Typing System -- Protocols for Tn-seq Analyses in The Group A Streptococcus -- Genetic Manipulation of Group A Streptococcus – Gene Deletion by Allelic Replacement -- Generation of Bioluminescent Group A Streptococcus for Biophotonic Imaging -- Whole Genome Sequence Analysis and Population Genomics of Group A streptococci -- Group A Streptococcus Transcriptome Analysis -- Protocol for Proteome Analysis of Group A Streptococcus -- Investigation of Group A Streptococcal Interactions with Host Glycan Structures using High Throughput Techniques: Glycan Microarray Analysis using Recombinant Protein and Whole Cells -- Using Lactococcus lactis as Surrogate Organism to Study Group A Streptococcus Surface Proteins -- Expression and Purification of Collagen-like Proteins of Group A Streptococcus -- Detection of Fibronectin-binding Proteins of Streptococcus pyogenes using Ligand Blot Analysis -- Morphology and Ultrastructure of Group A Streptococcus Biofilms -- Dynamic Interactions of Group A Streptococcus with Host Macrophages -- Identification of Group A Streptococcus-containing autophagosome-like vacuoles -- Flow Cytometry-based Assays to Quantify Complement Deposition and Neutrophil Uptake of Group a Streptococcus -- Live Cell Microscopy and Flow Cytometry to study Streptolysin S Mediated Erythrocyte Hemolysis -- Isolation of Monoclonal Antibodies to Group A Streptococcus Antigens Using Phage Display -- Assays to Analyse Adhesion of Group A Streptococcus to Host Cells -- The use of Galleria mellonella (wax moth) as an Infection Model for Group A Streptococcus -- A Superficial Skin Scarification Method in Mice to Mimic Streptococcus pyogenes Skin Infection in Humans -- A Mouse Nasopharyngeal Colonisation Model for Group A Streptococcus -- Humanised Plasminogen Mouse Model to Study Group A Streptococcus Invasive Disease -- Lacefield Whole Blood Killing Assay to Evaluate Vaccine Efficacy -- An Opsonophagocytic Killing Assay for the Evaluation of Group A Streptococcus Vaccine Antisera -- Validation of Suitable Carrier Molecules and Target Genes for antisense Therapy using Peptide-coupled Peptide Nucleic Acids (PNAs) in Streptococci -- Interfering with the Folding of Group A Streptococcal pili Proteins -- Use of Streptolysin O (SLO) to Study the Function of Lipid Rafts -- Engineering of Group A Streptococcus Isopeptide Bonds into Immunoglobulin-like Protein Domains. .
520 _aThis volume details Group A Streptococcus (GAS) research and provides the reader with an extensive collection of research protocols within this important field. Chapters guide readers through standard genetic protocols such as whole genome sequencing, transcriptome analyses, proteome analysis, application of GAS-derived molecular tools, and methods that are crucial for the evaluation of novel GAS vaccines and GAS virulence factors, including bactericidal assays and animal infection models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Group A Streptococcus: Methods and Protocols aims to ensure successful results in the further study of this vital field.
650 0 _aDiseases.
650 0 _aVirology.
650 1 4 _aDiseases.
650 2 4 _aVirology.
700 1 _aProft, Thomas.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aLoh, Jacelyn M. S.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071604663
776 0 8 _iPrinted edition:
_z9781071604687
776 0 8 _iPrinted edition:
_z9781071604694
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2136
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-0467-0
912 _aZDB-2-PRO
999 _c6099
_d6099