000 04863nam a22005175i 4500
001 978-1-0716-0648-3
003 DE-He213
005 20260610083640.0
007 cr nn 008mamaa
008 200420s2020 xxu| s |||| 0|eng d
020 _a9781071606483
_9978-1-0716-0648-3
024 7 _a10.1007/978-1-0716-0648-3
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
_223
245 1 0 _aMolecular Dermatology
_h[electronic resource] :
_bMethods and Protocols /
_cedited by ​Natalia V. Botchkareva, Gillian E. Westgate.
250 _a1st ed. 2020.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2020.
300 _aX, 278 p. 75 illus., 69 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2154
505 0 _aIsolation of Epidermal Keratinocytes from Human Skin: The Scratch-Wound Assay for Assessment of Epidermal Keratinocyte Migration -- Isolation of Different Dermal Fibroblast Populations from the Skin and the Hair Follicle -- Isolation of Epidermal and Hair Follicle Melanocytes -- Isolation and Culture of Human Skin Mast Cells -- Organotypic 3D Skin Models: Human Epidermal Equivalent Cultures from Primary Keratinocytes and Immortalized Keratinocyte Cell Lines -- Purification of Extracellular Microvesicles Secreted by Dermal Fibroblasts -- A Method to Investigate the Epidermal Permeability Barrier In Vitro -- Isolating Dermal Papilla Cells from Human Hair Follicles Using Microdissection and Enzyme Digestion -- Methods to Study Human Hair Follicle Growth Ex Vivo: Human Microdissected Hair Follicle and Human Full Thickness Skin Organ Culture -- Non-Surgical Induction of Alopecia Areata in C3H/HeJ Mice via Adoptive Transfer of Cultured Lymphoid Cells -- Collapse and Restoration of Hair Follicle ImmunePrivilege Ex Vivo: A Model for Alopecia Areata -- Experimentally-Induced Epithelial-Mesenchymal Transition of Human Hair Follicle Stem Cells as a Model of Scarring Alopecia Ex Vivo -- Generation of Hair Follicle Germs in Vitro Using Human Postnatal Skin Cells -- Method for Human Eccrine Sweat Gland Isolation from the Scalp by Means of the Micro-Punch Technique and Vital Dyes -- Identification of Long Non-Coding RNA by In Situ Hybridization Approaches -- Detection of MicroRNAs by In Situ Hybridization in Skin -- Chromatin Immunoprecipitation of Low Number of FACS-Purified Epidermal Cells -- 3D-FISH Analysis of the Spatial Genome Organization in Skin Cells In Situ -- Method to Study Skin Cancer: Two-Stage Chemically-Induced Carcinogenesis in Mouse Skin -- Investigation of Skin Wound Healing Using a Mouse Model -- Human Wound Healing Ex Vivo Model with Focus on Molecular Markers -- Method for Investigation of Photobiological Effects of Light on Human Skin Cells Mediated by Low Doses of Light.
520 _aThis volume delivers the most important laboratory techniques that underlie dermatological investigation, including in situ, in vitro, ex vivo, and in vivo models of skin, its appendages (hair follicles, sebaceous and sweat glands), and immune cells. Also included are unique techniques that unravel epigenetic mechanisms in skin and hair follicle cells, such as gene organization in the nucleus and gene expression regulation by the non-coding transcriptome, which are important recent developments in investigative dermatology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Molecular Dermatology: Methods and Protocols serves as an essential resource for researchers working in the areas of fundamental as well as applied dermatological science.
650 0 _aBiochemistry.
650 0 _aDermatology.
650 0 _aCytology.
650 1 4 _aBiochemistry.
650 2 4 _aDermatology.
650 2 4 _aCell Biology.
700 1 _aBotchkareva, ​Natalia V.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aWestgate, Gillian E.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071606476
776 0 8 _iPrinted edition:
_z9781071606490
776 0 8 _iPrinted edition:
_z9781071606506
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2154
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-0648-3
912 _aZDB-2-PRO
999 _c6098
_d6098