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020 _a9781071601679
_9978-1-0716-0167-9
024 7 _a10.1007/978-1-0716-0167-9
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
_223
245 1 0 _aInositol Phosphates
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Gregory J. Miller.
250 _a1st ed. 2020.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2020.
300 _aX, 185 p. 35 illus., 15 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2091
505 0 _aA Short Historical Perspective of Methods in Inositol Phosphate Research -- Quantitation of Inositol Phosphates by HPLC-ESI-MS -- Isolation of Inositol Hexakisphosphate from Soils by Alkaline Extraction and Hypobromite Oxidation -- Detection of Inositol Phosphates by Split PH Domains -- Dictyostelium discoideum as a Model to Study Inositol Pyrophosphate and Inorganic Polyphosphate -- Characterising Enzymes of the Diphosphoinositol Polyphosphate Phosphohydrolase (DIPP) Family -- Metabolic Labeling of Inositol Phosphates and Phosphatidylinositols in Yeast and Mammalian Cells -- Back-pyrophosphorylation Assay to Detect in vivo InsP7-dependent Protein Pyrophosphorylation in Mammalian Cells -- Analyses of Ligand Binding to IP3 receptors using Fluorescence Polarization -- Investigating the InsP3 Receptor in Living Cells by Caged InsP3 -- Optical Measurement of Synaptic Vesicle Fusion and its Inhibition by Inositol Pyrophosphate in Primary Cultured Hippocampal Neurons -- Competitive Fluorescent Ligand Assay for Inositol 1,4,5-Trisphosphate -- Studying the Effects of Inositol Pyrophosphates in an In Vitro Vesicle-vesicle Fusion Assay -- Engineering Protease Resistant and Highly Active Phytases -- Direct Cloning Method for Expression of Recombinant Proteins with an Inositol Hexakisphosphate Inducible Self-cleaving Tag -- A Short Historical Perspective of Methods in Inositol Phosphate Research. .
520 _aThis volume describes modern approaches for the detection and analysis of inositol phosphates from various biological, clinical, and environmental samples. Chapters focus on methods to study enzymes that modify inositol signals, chemical biology of inositol phosphates, and methods to synthesize and purify inositol phosphates and their analogs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Inositol Phosphates: Methods and Protocol aims to ensure successful results in the further study of this vital field.
650 0 _aBiochemistry.
650 0 _aCytology.
650 1 4 _aBiochemistry.
650 2 4 _aCell Biology.
700 1 _aMiller, Gregory J.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071601662
776 0 8 _iPrinted edition:
_z9781071601686
776 0 8 _iPrinted edition:
_z9781071601693
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2091
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-0167-9
912 _aZDB-2-PRO
999 _c6097
_d6097