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020 _a9781071601464
_9978-1-0716-0146-4
024 7 _a10.1007/978-1-0716-0146-4
_2doi
050 4 _aRC261-271
072 7 _aMJCL
_2bicssc
072 7 _aMED062000
_2bisacsh
072 7 _aMJCL
_2thema
082 0 4 _a571.978
_223
082 0 4 _a616.994
_223
245 1 0 _aChimeric Antigen Receptor T Cells
_h[electronic resource] :
_bDevelopment and Production /
_cedited by Kamilla Swiech, Kelen Cristina Ribeiro Malmegrim, Virgínia Picanço-Castro.
250 _a1st ed. 2020.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2020.
300 _aXI, 274 p. 29 illus., 25 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2086
505 0 _aCAR-T Cells for Cancer Treatment: Current Design and Next Frontiers -- Selection of Antibody Fragments for CAR-T Cell Therapy from Phage Display Libraries -- Phase I/II Manufacture of Lentiviral Vectors under GMP in an Academic Setting -- Production of Lentivirus for the Establishment of CAR-T Cells -- Optimized Production of Lentiviral Vectors for CAR-T Cell -- Lentiviral Vectors Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells -- In vitro Transcribed (IVT)-mRNA CAR Therapy Development -- Generation of Chimeric Antigen Receptor T Cells Using Gammaretroviral Vectors -- Generation of CAR+ T Lymphocytes using the Sleeping Beauty Transposon System -- Platforms for Clinical-grade Car-T Cell Expansion -- CAR-T Cell Expansion in a Xuri Cell Expansion System W25 -- Methods and Process Optimization for Large Scale CAR T Expansion Using the G-Rex Cell Culture Platform -- Replication Competent LentivirusAnalysis of Vector Transduced T Cell Products used in Cancer Immunotherapy Clinical Trials -- Immunophenotypic Analysis of CAR-T Cells -- Approaches of T Cell Activation and Differentiation for CAR-T Cell Therapies -- Determination of Cytotoxic Potential of CAR-T Cells in Co-cultivation Assays -- Analysis of CAR-mediated Tonic Signaling -- Generation of Tumor Cells Expressing Firefly Luciferase (fLuc) to Evaluate the Effectiveness of CAR in a Murine Model -- Analysis of Antitumor Effects of CAR-T Cells in Mice with Solid Tumors.
520 _aThis volume provides comprehensive methods from expert scientists working in the Chimeric Antigen Receptor T Cell (CAR-T Cell) field. Chapters guide readers through the state-of-art of CAR-T cell technology, CAR design and vector production, CAR-T cell generation and manufacturing, CAR-T cell characterization, and quality control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chimeric Antigen Receptor T Cells: Development and Production aims to be useful in the production of CAR-T cells, especially for therapeutic purposes.
650 0 _aCancer.
650 0 _aCytology.
650 0 _aImmunology.
650 0 _aPharmacology.
650 1 4 _aCancer Biology.
650 2 4 _aCell Biology.
650 2 4 _aImmunology.
650 2 4 _aPharmacology.
700 1 _aSwiech, Kamilla.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aMalmegrim, Kelen Cristina Ribeiro.
_eeditor.
_0(orcid)0000-0002-9303-0086
_1https://orcid.org/0000-0002-9303-0086
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aPicanço-Castro, Virgínia.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071601457
776 0 8 _iPrinted edition:
_z9781071601471
776 0 8 _iPrinted edition:
_z9781071601488
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2086
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-0146-4
912 _aZDB-2-PRO
999 _c6096
_d6096