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020 _a9781071601389
_9978-1-0716-0138-9
024 7 _a10.1007/978-1-0716-0138-9
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572
_223
245 1 0 _aNucleic Acid Detection and Structural Investigations
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Kira Astakhova, Syeda Atia Bukhari.
250 _a1st ed. 2020.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2020.
300 _aXI, 270 p. 72 illus., 48 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2063
505 0 _aOptomagnetic Detection of Rolling Circle Amplification Products -- Subtyping of Swine Influenza Using a High-Throughput Real-Time PCR Platform and a Single Microfluidics Device -- RT-qPCR Detection of Low Copy HIV RNA with Yin-Yang Probes -- Solid-phase Hybridization Assay for Detection of Mutated Cancer DNA by Fluorescence -- 5'-Mono- and 5'-bis-pyrene 2'-O-methyl RNA Probes for Detection of RNA Mismatches -- Combined Assay for Detecting Autoantibodies to Nucleic Acids and Apolipoprotein H in Patients with Systemic Lupus Erythematosus -- Electrochemical Detection of dsDNA-specific Antibodies -- Sequence-defined DNA-amphiphiles for Drug Delivery: Synthesis and Self-assembly -- DNA-mediated Liposome Fusion Observed by Fluorescence Spectrometry -- Advanced Fluorescence Imaging to Distinguish Between Intracellular Fractions of Antisense Oligonucleotides -- Methodology for Subcellular Fractionation and microRNA Examination of Mitochondria, Mitochondria Associated ER Membrane (MAM), ER, and Cytosol from Human Brain -- Skeletal Muscle Injury by Electroporation – A Model to Study Degeneration/Regeneration Pathways in Muscle -- Isolation and Characterization of Muscle Derived Stem Cells from Dystrophic Mouse Models -- Universal Library Preparation Protocol for Efficient High-Throughput Sequencing Of Double-stranded RNA Viruses -- Quantitation of Molecular Pathway Activation using RNA Sequencing Data -- Molecular Pathway Analysis of Mutation Data for Biomarkers Discovery and Scoring of Target Cancer Drugs -- Oncobox Method for Scoring Efficiencies of Anticancer Drugs Based on Gene Expression Data -- Global Characterization of Circulating Nucleic Acids. .
520 _aThis book volume provides in depth coverage of the nucleic acid field and aims to represent a broad diversity of the methodologies and a wide range of nucleic-acid-related topics within the fields of molecular biology and biomedicine. Chapters are divided in three sections detailing in vitro detection, nanotechnology and imaging, biomedical applications, and big data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nucleic Acid Detection and Structural Investigations: Methods and Protocols aims present aims to present methodologies combine cutting-edge innovation with sound theory and practical applications in life sciences.
650 0 _aBiochemistry.
650 1 4 _aBiochemistry.
700 1 _aAstakhova, Kira.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBukhari, Syeda Atia.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071601372
776 0 8 _iPrinted edition:
_z9781071601396
776 0 8 _iPrinted edition:
_z9781071601402
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2063
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-0138-9
912 _aZDB-2-PRO
999 _c6095
_d6095