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024 7 _a10.1007/978-1-0716-5154-4
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245 1 0 _aLaser Capture Microdissection
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Roberta Noberini.
250 _a1st ed. 2026.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2026.
300 _aXI, 213 p. 50 illus., 48 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bPDF/UA-1
_2onix
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v3015
505 0 _aLaser capture microdissection from blood smears -- Live cell isolation by laser capture microdissection -- From Laser Microdissection to Spatial Glycomics: Lectin Microarray Protocols for Tissue Glycome Mapping with High-End Scanners -- Laser capture microdissection followed by DNA methylation profiling -- High resolution laser microdissection to investigate transcriptional states in formalin-fixed paraffin embedded samples -- Transcriptomic analysis from cryosectioned tissue samples using laser capture microdissection based Geo-seq -- Immunofluorescence imaging-guided laser microdissection for ultra-low input spatial tissue proteomics -- Low-Input Proteomics Protocols for Laser-Captured FFPE Samples with Orbitrap Astral DIA-MS -- Laser capture microdissection followed by histone H1 variant analysis by mass spectrometry -- A spatial proteomics approach to isolate and analyze amyloid proteins using laser capture microdissection and mass spectrometry -- Chromatin Immunoprecipitation-Sequencing and RNA-Sequencing from Laser Microdissected Human Lung Cancer Cells -- Laser capture microdissection followed by ATAC-seq -- Laser Capture Microdissection for quantitative shotgun lipidomics -- Artificial Intelligence-driven laser capture microdissection -- Automated Intelligent Microscopy for phenotypic identification, spatial localization and retargeting of cells.
520 _aThis volume details protocols that highlight the versatility of LCM across different biological contexts and analytical workflows. Chapters cover protocols on isolating cells from blood smears, live cell samples, and FFPE or frozen tissues. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Laser Capture Microdissection: Methods and Protocols aims to support both new and experienced researchers in effectively applying LCM to their own biological questions, across a wide variety of systems and technical platforms.
532 8 _aAccessibility summary: This PDF has been created in accordance with the PDF/UA-1 standard to enhance accessibility, including screen reader support, described non-text content (images, graphs), bookmarks for easy navigation, keyboard-friendly links and forms and searchable, selectable text. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com. Please note that a more accessible version of this eBook is available as ePub.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aMaterials
_xMicroscopy.
650 0 _aImaging systems in biology.
650 0 _aMaterials
_xAnalysis.
650 0 _aImaging systems.
650 1 4 _aMicroscopy.
650 2 4 _aBiological Imaging.
650 2 4 _aImaging Techniques.
700 1 _aNoberini, Roberta.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071651537
776 0 8 _iPrinted edition:
_z9781071651551
776 0 8 _iPrinted edition:
_z9781071651568
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v3015
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-5154-4
912 _aZDB-2-PRO
999 _c6071
_d6071