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020 _a9781071651469
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024 7 _a10.1007/978-1-0716-5146-9
_2doi
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082 0 4 _a579.028
_223
245 1 0 _aEuglenozoa
_h[electronic resource] :
_bMethods and Protocols, Volume 2 /
_cedited by Paul A.M. Michels, Michael L. Ginger, Anna Karnkowska, Laura-Isobel McCall, Ariel M. Silber.
250 _a1st ed. 2026.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2026.
300 _aXVI, 419 p. 51 illus., 47 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bPDF/UA-1
_2onix
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v3014
505 0 _aGenetic Manipulation of Paradiplonema papillatum -- Protocol for Efficient Ribodepletion of Euglena gracilis RNA -- Engineering Wax Ester Composition in Euglena gracilis Using Genome Editing -- Multi-Approach Workflows for Chromatin and Histone Isolation from Trypanosoma cruzi: From Basic Protein Extraction to Mass Spectrometry-Compatible Protocols -- Precision Without Selection: A Marker-Free CRISPR/Cas9-Based Protocol for Multiplexed Genome Editing in Trypanosomatids -- Application of Double-Inducible Expression System in Bloodstream-Form Trypanosoma brucei -- Tetracycline-Inducible Functional Complementation Analysis in Trypanosoma Parasites -- Immunoprecipitation of Protein Complexes from Trypanosoma brucei -- Standardized Recombinase Polymerase Amplification (RPA) Assay for Detecting Active Trypanosoma brucei evansi Infections -- High Throughput Cell Cycle and Morphological Analysis of Leishmania mexicana and Other Kinetoplastids -- Protein Localization with Streptavidin-Imaging: Boost Your Signal, Reduce Noise, Overcome Antibody Accessibility Problems, and Time-Travel to the Past -- A Practical Guide to Ultrastructural Expansion Microscopy (U-ExM) in Trypanosoma brucei -- Expansion Microscopy in Euglenozoa -- Colab-PEX19BS: An Automated Google Colab Notebook-Based Tool for Detecting PEX19 Binding Sites -- Biochemistry of Diplonemids -- Euglena gracilis Subcellular Fractionation -- Isolation of Mitochondria and Chloroplasts from Euglena gracilis Z -- Imaging Ca2+ Signaling in Trypanosoma cruzi with Genetically-Encoded Ca2+ Indicators -- Enzyme Assays for Phosphoinositide Kinases and Phosphatases and Inhibitor Screens -- Non-Invasive, Fluorescence-Based Detection of Perturbations in the Thiol-Redox Homeostasis of Bloodstream Trypanosoma brucei brucei -- Assessment of Mitochondrial Membrane Potential in Intact and Detergent-Permeabilized Trypanosoma brucei Insect and Bloodstream Forms -- Camelid Single-Domain Antibodies as Tools for Novel Target Identification in Kinetoplastid Research -- Method for Purification and Electrophysiological Recordings of Ion Channels from Trypanosomatids -- Functional Studies of Kinetoplastid Transporters.
520 _aThis detailed collection covers methods recently developed for experiments on organisms from each of the major euglenozoan groups, including diplonemids, euglenids, and kinetoplastids. Volume 2 presents three sections exploring techniques in molecular biology, cell biology, as well as biochemistry and physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Euglenozoa: Methods and Protocols, Volume 2 serves as an ideal guide for researchers working with these fascinating organisms and investigating the aspects of their extreme biology. Chapters 21 and 22 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
532 8 _aAccessibility summary: This PDF has been created in accordance with the PDF/UA-1 standard to enhance accessibility, including screen reader support, described non-text content (images, graphs), bookmarks for easy navigation, keyboard-friendly links and forms and searchable, selectable text. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com. Please note that a more accessible version of this eBook is available as ePub.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aMicrobiology
_xTechnique.
650 0 _aParasitology.
650 1 4 _aMicrobiology Techniques.
650 2 4 _aParasitology.
700 1 _aMichels, Paul A.M.
_eeditor.
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_1https://orcid.org/0000-0003-3726-6104
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700 1 _aGinger, Michael L.
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700 1 _aKarnkowska, Anna.
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700 1 _aMcCall, Laura-Isobel.
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700 1 _aSilber, Ariel M.
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710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
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776 0 8 _iPrinted edition:
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776 0 8 _iPrinted edition:
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830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
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856 4 0 _uhttps://doi.org/10.1007/978-1-0716-5146-9
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