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020 _a9781071651421
_9978-1-0716-5142-1
024 7 _a10.1007/978-1-0716-5142-1
_2doi
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082 0 4 _a579.028
_223
245 1 0 _aEuglenozoa
_h[electronic resource] :
_bMethods and Protocols, Volume 1 /
_cedited by Paul A.M. Michels, Michael L. Ginger, Anna Karnkowska, Laura-Isobel McCall, Ariel M. Silber.
250 _a1st ed. 2026.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2026.
300 _aXIV, 412 p. 49 illus., 47 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bPDF/UA-1
_2onix
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v3013
505 0 _aWhy Euglenozoans -- Isolation and Cultivation of Diplonemids -- Preparation of Diplonemid Samples for Microscopy -- Protocol for the Molecular Identification of Autotrophic Euglenids in Environmental Samples -- Culture and Genetic Modification of the Monoxenous Trypanosomatids Crithidia fasciculata and Crithidia bombi -- Poly(A) Profiling for Euglenozoans Using Oxford Nanopore Direct RNA Sequencing, Illustrated by the Example of Euglena gracilis -- Euglenid Extrachromosomal DNA: Assembly and Annotation -- Nuclear Genome Assembly and Annotation of Kinetoplastids -- Assembly and Annotation of Kinetoplastid and Diplonemid Mitochondrial Genomes -- Cleavage Under Targets and Release Using Nuclease (CUT&RUN) for Trypanosoma brucei Parasites -- Mapping Open Chromatin in Trypanosoma brucei Using ATAC-Seq -- Transcriptomic Analysis of Trypanosomatids -- Small RNA Profiling in Trypanosomes -- Desorption Electrospray Ionization–Mass Spectrometry Imaging (DESI-MSI) of Metabolites in Heart Samples Infected with Trypanosoma cruzi -- Microbiome Profiling in Chagas Disease: Sample Collection, Sequencing, and Analysis -- Metabolomics Analysis of Trypanosomes Using Ion Mobility-Enhanced Mass Spectrometry -- Exploring Protein Interactomes Using TurboID-Directed Proximity Labeling and Mass Spectrometry -- Mapping Trypanosoma Protein Interactome by Proximity-Dependent Biotinylation -- From Genome to Function: Metabolism-Wide Models of Lifecycle Stages of Parasites -- Enhanced Detection of Homology Using Artificial Intelligence in Euglenids.
520 _aThis detailed collection covers methods recently developed for experiments on organisms from each of the major euglenozoan groups, including diplonemids, euglenids, and kinetoplastids. Volume 1 presents a historical review of the research into euglenozoans, followed by sections of techniques involving isolation and culturing of different species of euglenozoans, as well as a variety of -omics research methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Euglenozoa: Methods and Protocols, Volume 1 serves as an ideal guide for researchers working with these fascinating organisms and investigating the aspects of their extreme biology. Chapter 14 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
532 8 _aAccessibility summary: This PDF has been created in accordance with the PDF/UA-1 standard to enhance accessibility, including screen reader support, described non-text content (images, graphs), bookmarks for easy navigation, keyboard-friendly links and forms and searchable, selectable text. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com. Please note that a more accessible version of this eBook is available as ePub.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aMicrobiology
_xTechnique.
650 0 _aParasitology.
650 1 4 _aMicrobiology Techniques.
650 2 4 _aParasitology.
700 1 _aMichels, Paul A.M.
_eeditor.
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700 1 _aGinger, Michael L.
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700 1 _aKarnkowska, Anna.
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700 1 _aMcCall, Laura-Isobel.
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700 1 _aSilber, Ariel M.
_eeditor.
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710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
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776 0 8 _iPrinted edition:
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776 0 8 _iPrinted edition:
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830 0 _aMethods in Molecular Biology,
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856 4 0 _uhttps://doi.org/10.1007/978-1-0716-5142-1
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