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020 _a9781071634578
_9978-1-0716-3457-8
024 7 _a10.1007/978-1-0716-3457-8
_2doi
050 4 _aQD431-431.7
072 7 _aPSBC
_2bicssc
072 7 _aSCI013040
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572.6
_223
245 1 0 _aMass Spectrometry-Based Proteomics
_h[electronic resource] /
_cedited by Kris Gevaert.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXI, 336 p. 66 illus., 57 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2718
505 0 _a1. Increasing the Overall Proteome Coverage by Combining Protein Digestion by Tryp-N and Trypsin -- Cell Surface Biotinylation Using Furan Crosslinking Chemistry -- Studying Cellular Dynamics Using Proximity-Dependent Biotinylation: Somatic Cell Reprogramming -- Virotrap: Trapping Protein Complexes in Virus-Like Particles -- Thermal Proteome Profiling for Drug Target Identification and Probing of Protein States -- Improved Coverage of the N-Terminome by Combining ChaFRADIC with Alternative Proteases -- Sensitive and High-Throughput Exploration of Protein N Termini by TMT-TAILS N-Terminomics -- The Global Acetylation Profiling Pipeline for Quick Assessment of Protein N-Acetyltransferase Specificity In Cellulo -- Systems-Wide Site-Specific Analysis of Glycoproteins -- Targeted Profiling of Protein Phosphorylation in Plants -- Automated Sample Preparation for Mass Spectrometry-Based Clinical Proteomics -- Proteomics-Based Analysis and Diagnosis of Formalin-Fixed Paraffin-Embedded Amyloidosis Samples -- A Robust and Clinically-Applicable Sample Preparation Protocol for Urinary Extracellular Vesicle Isolation Suitable for Mass Spectrometry-Based Proteomics -- Density-Based Fractionation of Cell Conditioned Medium to Prepare Proteomics Grade Extracellular Vesicles -- Mass Spectrometry-Based Analysis of Histone Post-Translational Modifications from Laser Microdissected Samples -- Phosphoproteomics after Guanidinium Thiocyanate Extraction of Tissue Biopsies -- Probing Antibody Structures by Hydrogen/Deuterium Exchange Mass Spectrometry.
520 _aThis detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by proteases, protein N-glycosylation, and protein phosphorylation. The book also collects chapters on automated preparation of clinical samples, the analysis of formalin-fixed paraffin-embedded samples, protocols for the isolation of extracellular vesicles and for the monitoring of selected protein modifications in clinical samples, and, finally, structural proteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mass Spectrometry-Based Proteomics serves as an ideal guide to its subject for both novices in the field of proteomics as well as specialists.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aProteins.
650 0 _aMass spectroscopy.
650 1 4 _aProteins.
650 2 4 _aMass Spectrometry.
700 1 _aGevaert, Kris.
_eeditor.
_0(orcid)0000-0002-4237-0283
_1https://orcid.org/0000-0002-4237-0283
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071634561
776 0 8 _iPrinted edition:
_z9781071634585
776 0 8 _iPrinted edition:
_z9781071634592
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2718
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3457-8
912 _aZDB-2-PRO
999 _c6068
_d6068