000 04933nam a22005775i 4500
001 978-1-0716-3004-4
003 DE-He213
005 20260610083640.0
007 cr nn 008mamaa
008 230228s2023 xxu| s |||| 0|eng d
020 _a9781071630044
_9978-1-0716-3004-4
024 7 _a10.1007/978-1-0716-3004-4
_2doi
050 4 _aQH426-470
072 7 _aPSAK
_2bicssc
072 7 _aSCI029000
_2bisacsh
072 7 _aPSAK
_2thema
082 0 4 _a576.5
_223
245 1 0 _aDNA Manipulation and Analysis
_h[electronic resource] /
_cedited by Garry Scarlett.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aX, 249 p. 49 illus., 44 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2633
505 0 _aClassical Recombinant DNA Cloning -- A Sequence and Ligation-Independent Cloning (SLIC) Procedure for the Insertion of Genes into a Plasmid Vector -- Molecular cloning using in vivo DNA assembly -- Assembling multiple fragments: The Gibson Assembly -- TA cloning approaches to cloning DNA with damaged ends DNA -- PCR-based Assembly of Gene Sequences by Thermodynamically Balanced Inside-Out (TBIO) Gene Synthesis -- Random Mutagenesis by PCR -- In vitro Site Directed Mutagenesis -- Xenopus transgenesis using the pGateway system -- CRISPR/Cas9 gene disruption studies in F0 Xenopus tadpoles: understanding development and disease in the frog -- A CRISPR/Cas based method for precise DNA integration in Xenopus laevis oocytes followed by intracytoplasmic sperm injection (ICSI) fertilisation -- A Lambda-Exonuclease SELEX Method for Generating Aptamers to Bacterial Targets. -Generation of functional-RNA arrays by in vitrotranscription and in situ RNA capture for the detection of RNA-RNA interactions -- Chemical synthesis of oligonucelotide sequences: Phosphoramidite chemistry -- Low Throughput Direct Cycle Sequencing of Polymerase Chain Reaction (PCR) Products -- Next generation Sequencing for mixed samples -- Ethics, Legality and Safety for Geneticists.
520 _aThis volume details technologies on recombinant DNA and nucleic acid manipulation that underpin much of the biological sciences and instructions on how to conduct them successfully. Chapters guide the reader through the basics of oligonucleotide synthesis and DNA sequencing; recombinant DNA plasmid work; cell-free experiments and the latest developments in CRISPR approaches to genome modification. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, DNA Manipulation and Analysis aims to be the comprehensive guide for life scientists moving into the field of recombinant DNA and nucleic acid manipulation.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aGenetics.
650 1 4 _aGenetics and Genomics.
700 1 _aScarlett, Garry.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071630037
776 0 8 _iPrinted edition:
_z9781071630051
776 0 8 _iPrinted edition:
_z9781071630068
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2633
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3004-4
912 _aZDB-2-PRO
999 _c6066
_d6066