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020 _a9781071629468
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024 7 _a10.1007/978-1-0716-2946-8
_2doi
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
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082 0 4 _a572
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245 1 0 _aProteoglycans
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Nikos K. Karamanos.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXIV, 317 p. 67 illus., 59 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2619
505 0 _aDetection of Glycosaminoglycans in Biological Specimens -- Immunochemical Detection and Glycosaminoglycan Disaccharide-Based Characterization Of Chondroitin Sulfate Proteoglycans -- Proteoglycans: A Tool for detecting hyaluronan by ELISA-like Methods -- Particle Exclusion Assay: A Tool for Measuring Hyaluronan Pericellular Matrix -- Urinary Glycosaminoglycans: Characterization and Quantification -- HPLC-MS characterization of tissue-derived heparan sulfate and chondroitin sulfate -- Evaluation of Glycosaminoglycans Biological Age in Cells and Tissues -- Studying the effects of Glycosaminoglycans in Cell Morphological Aspect With Scanning Electron Microscopy -- Evaluation of the In Vitro and In Vivo Effects of Biglycan in Innate Immunity -- Analysis of Extracellular Vesicle-associated Proteoglycans -- Analysis of Aggrecan Glycanation by Western Blot in Cell Culture -- Repulsive Scaling Replica Exchange Molecular Dynamics in ModelingProtein-Glycosaminoglycan Complexes -- In vitro investigation of hyaluronan/CD44 network -- Separation and Identification of Native Proteoglycans by Composite Agarose-Polyacrylamide Gel Electrophoresis and Immunoblotting -- Building, Visualizing, and Analyzing Glycosaminoglycan-Protein Interaction Networks -- An Enzymatic Activity Assay for Heparanase That Is Useful for Evaluating Clinically Relevant Inhibitors and Studying Kinetics -- A method for Urinary Trypsin Inhibitor (UTI) purification combining anion-exchange chromatography enrichment and preparative SDS-PAGE -- Enzymatic Sequencing of Heparin Oligosaccharides Using Exolyase -- Evaluating the Effects of microRNAs on Proteoglycans and Matrix Constituents’ Expression and Functional Properties -- Proteoglycan expression studied by microRNAs -- Modeling and simulations of Extra Cellular Glycoproteins.
520 _aThis volume is a comprehensive and up-to-date collection of strategies, reproducible methods, and protocols for the in-depth analysis of Proteoglycans (PGs) and their glycan part, the GAGs. Chapters are divided into three parts detailing GAGs in biological specimens, protocols for the evaluation of the in vitro and in vivo effects of PGs/GAGs, and protocols for compounds related with the metabolic enzymes, epigenetic regulation, and PGs/GAGs-based inhibitors. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Cutting-edge and thorough, Proteoglycans: Methods and Protocols aims to provide information on the elucidated the structural and functionalaspects of the complex matrix macromolecules such as the proteoglycans and glycosaminoglycans. .
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBiochemistry.
650 0 _aProteins.
650 1 4 _aBiochemistry.
650 2 4 _aProteins.
700 1 _aKaramanos, Nikos K.
_eeditor.
_0(orcid)0000-0003-3618-0288
_1https://orcid.org/0000-0003-3618-0288
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071629451
776 0 8 _iPrinted edition:
_z9781071629475
776 0 8 _iPrinted edition:
_z9781071629482
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2619
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2946-8
912 _aZDB-2-PRO
999 _c6065
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