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020 _a9781071601839
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024 7 _a10.1007/978-1-0716-0183-9
_2doi
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_2bicssc
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072 7 _aSCI011000
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082 0 4 _a571.82
_223
245 1 0 _aPlant Stem Cells
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Muhammad Naseem, Thomas Dandekar.
250 _a1st ed. 2020.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2020.
300 _aXI, 150 p. 40 illus., 37 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2094
505 0 _aRegulatory Role of Phytohormones in Maintaining Stem Cells and Boundaries of Stem Cell Niches -- Bacterial Shoot Apical Meristem Inoculation Assay -- Germline-Transmitted Genome Editing Methodology in Arabidopsis thaliana using TAL-Effectors Nucleases -- Method to Study Gene Expression Patterns during de novo Root Regeneration from Arabidopsis Leaf Explants -- Labeling and Sorting of Arabidopsis SAM Cell Populations to Capture their Transcriptome Profile -- Plant Associated Microbes Alter Root Growth by Modulating Root Apical Meristem -- Live Imaging of Arabidopsis Axillary Meristems -- Molecular Modeling of the Interaction between Stem Cells Peptide and Immune Receptor in Arabidopsis -- Methods to Visualize Auxin and Cytokinins Signaling Activity in the Shoot Apical Meristem -- Analysis of Cell Division Frequency in the Root Apical Meristem of Lycophytes, Non-Seed Vascular Plants, using EdU Labeling -- Osmotic Treatment for Quantifying Cell WallElasticity in the Sepal of Arabidopsis thaliana -- Mapping a Transcriptome Guided Arabidopsis SAM Interactome -- 3D Analysis of Mitosis Distribution Pattern in the Plant Root Tip with iRoCS Toolbox -- Micropropagation of Rosaceous Species SAM Grown in Temperate Climate -- A New Perspective on Cryotherapy: Pathogen Elimination using Plant Shoot Apical Meristem via Cryogenic Techniques.
520 _aThis volume looks at the latest technologies and methods used by researchers to study mechanisms that control the aspects of plant stem cell signaling events. The topics discussed in this book cover shoot apical meristem inoculation assay; plant associated microbes that alter root growth by modulating root apical meristem; visualizing auxin and cytokinins signaling activity; 3D analysis of mitosis distribution pattern in the plant root tip with iRoCS toolbox; and micropropagation of rosaceous species SAM grown in temperate climate. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Plant Stem Cells: Methods and Protocols is a useful resource for all researchers interested in learning more about this developing field.
650 0 _aPlants
_xDevelopment.
650 0 _aStem cells.
650 1 4 _aPlant Development.
650 2 4 _aStem Cell Biology.
700 1 _aNaseem, Muhammad.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aDandekar, Thomas.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071601822
776 0 8 _iPrinted edition:
_z9781071601846
776 0 8 _iPrinted edition:
_z9781071601853
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2094
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-0183-9
912 _aZDB-2-PRO
999 _c6015
_d6015