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020 _a9781627035507
_9978-1-62703-550-7
024 7 _a10.1007/978-1-62703-550-7
_2doi
050 4 _aQH573-671
072 7 _aPSF
_2bicssc
072 7 _aSCI017000
_2bisacsh
072 7 _aPSF
_2thema
082 0 4 _a571.6
_223
245 1 0 _aImmobilization of Enzymes and Cells
_h[electronic resource] /
_cedited by Jose M. Guisan.
250 _a3rd ed. 2013.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c2013.
300 _aXI, 377 p. 116 illus., 39 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1051
505 0 _aNew Opportunities for Immobilization of Enzymes -- Immobilization of Enzymes: A Literature Survey -- Glutaraldehyde Mediated Protein Immobilization -- Immobilization of Enzymes on Mono-and Hetero-Functional Epoxy-Activated Supports -- Stabilization of Enzymes by Multipoint Covalent Immobilization on Supports Activated with Glyoxyl Groups -- Oriented Covalent Immobilization of Enzymes on Heterofunctional-Glyoxyl Supports -- Reversible Covalent Immobilization of Enzymes Via Disulfide Bonds -- Immobilization of Candida rugosa Lipase on Superparamagnetic Fe3O4 Nanoparticles for Biocatalysis in Low Water Media -- Immobilization of Enzymes by Bioaffinity Layering -- Immobilization of Enzymes on Magnetic Beads through Affinity Interactions -- Tips for the Functionalization of  Nanoparticles with Antibodies -- Design and Characterization of Functional Nanoparticles for Enhanced Bio-Performance -- Immobilization of Enzymes on Ethynyl-modified Electrodes Via Click Chemistry -- Modification of Carbon Nanotube Electrodes with 1-pyrenebutanoic Acid, Succinimidyl Ester for Enhanced Bioelectrocatalysis -- Enzyme Immobilization by Entrapment within a Gel Network -- Practical Protocols for Lipase Immobilization via Sol-Gel Techniques -- Improving Lipase Activity by Immobilization and Post-Immobilization Strategies -- High Activity Preparations of Lipases and Proteases for Catalysis in Low Water Containing Organic Solvents and Ionic Liquids -- Biomedical Applications of Immobilized Enzymes: An Update -- Immobilization of Whole Cells by Chemical Vapor Deposition of Silica -- Encapsulation of Cells in Alginate Gels -- Microalgal Immobilization Methods -- Therapeutic Applications of Encapsulated Cells -- Whole Cell Entrapment Techniques.
520 _aFor most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols,  in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.
650 0 _aCytology.
650 0 _aEnzymology.
650 1 4 _aCell Biology.
650 2 4 _aEnzymology.
700 1 _aGuisan, Jose M.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781627035491
776 0 8 _iPrinted edition:
_z9781627035514
776 0 8 _iPrinted edition:
_z9781493959884
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v1051
856 4 0 _uhttps://doi.org/10.1007/978-1-62703-550-7
912 _aZDB-2-PRO
999 _c5986
_d5986