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020 _a9781071632956
_9978-1-0716-3295-6
024 7 _a10.1007/978-1-0716-3295-6
_2doi
050 4 _aQH426-470
072 7 _aPSAK
_2bicssc
072 7 _aSCI029000
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072 7 _aPSAK
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082 0 4 _a576.5
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245 1 0 _aForensic DNA Analysis
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Catherine Cupples Connon.
250 _a1st ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aIX, 431 p. 95 illus., 92 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2685
505 0 _aForensic DNA Analysis: An Overview of the Laboratory Process -- Organic Extraction of Nucleic Acids using Ethanol Precipitation or Microcon® Centrifugal Filter Purification Methods -- Manual Silica-Based DNA Extractions -- Applied Biosystems™ PrepFiler™ Forensic DNA Extraction Kit (Manual and Semi-Automated via AutoMate Express™) -- Robotic DNA Extraction Utilizing Qiagen BioSprint® 96 Workstation -- DNA Extraction of Bone through Demineralization -- Differential Extraction with Purification via Organic/Microcon® and Promega DNA IQ™ Methods -- DNA Purification from Bloodstains and Buccal Cells/Saliva on FTA® Cards -- Yield Gel via Quantitative Gel Electrophoresis -- Quantitative PCR of Alu Repeats using PowerUp™ SYBR® Green Master Mix -- Quantitation of DNA using the Applied Biosystems Quantifiler® Trio DNA Quantification Kit -- QIAGEN's Investigator® Quantiplex Pro Kit -- DNA Amplification using Promega's PowerPlex® Fusion Systems (5C and 6C) -- Amplification of Extracted DNA and Direct Amplification with the PowerPlex® Y23 System -- Applied Biosystems' GlobalFiler™ PCR Amplification Kit -- QIAGEN’s Investigator® 24plex QS and GO! PCR Amplification -- Low Volume STR Amplification Options: Coupling with Standard or Fast PCR, Traditional or Normalized DNA Extraction, and/or Traditional or Alternative Capillary Electrophoresis -- Capillary Electrophoresis with Applied Biosystems' 3500 Genetic Analyzer -- Likelihood Ratio Calculation using LRmix Studio -- Mitochondrial DNA Analysis -- An Optimized Forensic DNA Analysis Workflow for Obtaining STR Results from Archived Latent Fingerprints -- Detection of Latent DNA using a DNA Binding Dye -- Rapid DNA Profile Development with Applied Biosystems RapidHIT™ ID System -- Next-Generation Sequencing: ForenSeq™ DNA Signature Prep Kit with the Illumina MiSeq FGx.
520 _aThis volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures using both home-brew and commercial products. The chapters also discuss probabilistic modeling software and specialized start-to-finish procedures for mitochondrial DNA analysis, archived latent fingerprints, latent DNA, rapid DNA profiling, and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchersinterested in learning more about forensic DNA analysis procedures.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aGenetics.
650 0 _aMedical jurisprudence.
650 1 4 _aGenetics and Genomics.
650 2 4 _aForensic Medicine.
700 1 _aCupples Connon, Catherine.
_eeditor.
_0(orcid)0000-0002-4556-5633
_1https://orcid.org/0000-0002-4556-5633
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071632949
776 0 8 _iPrinted edition:
_z9781071632963
776 0 8 _iPrinted edition:
_z9781071632970
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2685
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3295-6
912 _aZDB-2-PRO
999 _c5965
_d5965