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024 7 _a10.1007/978-1-0716-3243-7
_2doi
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245 1 0 _aBacterial Pathogenesis
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Pontus Nordenfelt, Mattias Collin.
250 _a2nd ed. 2023.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2023.
300 _aXIV, 337 p. 70 illus., 66 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
341 0 _bTable of contents navigation
_2onix
341 0 _bSingle logical reading order
_2onix
341 0 _bShort alternative textual descriptions
_2onix
341 0 _bUse of color is not sole means of conveying information
_2onix
341 0 _bUse of high contrast between text and background color
_2onix
341 0 _bNext / Previous structural navigation
_2onix
341 0 _bAll non-decorative content supports reading without sight
_2onix
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2674
505 0 _aMeasuring Niche-Associated Metabolic Activity in Planktonic and Biofilm Bacteria -- Formation and Analysis of Mono-Species and Polymicrobial Oral Biofilms in Flow-Cell Models -- Isolation and Purification of Mycobacterial Extracellular Vesicles (EVs) -- Strategies to Isolate Extracellular Vesicles from Gram - and Gram + Bacteria -- Total Bacterial RNA Isolation and Northern Blotting Analysis -- Phylogenetic Analysis of Bacterial Pathogen Genomes -- Determination of Growth Rate and Virulence Plasmid Copy Number during Yersinia pseudotuberculosis Infection Using Droplet Digital PCR -- Methods to Analyze the Contribution of Complement Evasion Factor (CEF) to Streptococcus pyogenes Virulence -- Expression of the Bacterial Enzyme IdeS Using a GFP Fusion in the Yeast Saccharomyces cerevisiae -- Mass Spectrometry-Based Methods to Determine the Substrate Specificities and Kinetics of N-Linked Glycan Hydrolysis by Endo-β-N-Acetylglucosaminidases -- Identification of Substrates of Secreted Bacterial Protease by APEX2-Based Proximity Labeling -- Affinity-Purification Combined with Crosslinking Mass Spectrometry for Identification and Structural Modeling of Host-Pathogen Protein-Protein Complexes -- Elucidating the Stoichiometries of Host-Pathogen Protein Interactions with Mass Photometry -- Analysis of Neutrophil and Monocyte Inflammation Markers in Response to Gram-Positive Anaerobic Cocci -- Quantification of Phagocytosis Using Flow Cytometry -- Anti-Bacterial Neutrophil Effector Response: Ex Vivo Quantification of Regulated Cell Death Associated with Extracellular Trap Release -- Measurement of Antibody Binding Affinity on Bacterial Surfaces Using Flow Cytometry -- Detection of Inflammasome Activation in Murine Bone Marrow-Derived Macrophages Infected with Group A Streptococcus -- In Vivo Profiling of the Vascular Cell Surface Proteome in Murine Models of Bacteremia -- Barcoded Consortium Infections: A Scalable, Internally Controlled Method to Study Host Cell Binding and Invasion by Pathogenic Bacteria -- A Murine Mycobacterium marinum Infection Model for Longitudinal Analyses of Disease Development and the Inflammatory Response -- In Vitro Approaches for the Study of Pneumococcal-Neuronal Interaction and Pathogenesis.
520 _aThis detailed volume presents a diverse set of methodological approaches designed to improve our understanding of bacterial infections from a wide range of bacterial species. Beginning with biofilms and subcellular compartments, the book explores transcriptional analysis, methods for studying plasmid dynamics, and tools for phylogenetic analysis of bacterial genomes, as well as bacterial effector proteins interfering with host systems, host response analysis, and in vivo and in vitro infection models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Bacterial Pathogenesis: Methods and Protocols, Second Edition is a vital resource for researchers in the area of infection biology, as well as but not limited to, those working in the fields of microbiology, immunology, structural biology, molecular biology, genetics, imaging, and computational study.
532 8 _aAccessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com.
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aBacteria.
650 0 _aDiseases
_xCauses and theories of causation.
650 1 4 _aBacteria.
650 2 4 _aPathogenesis.
700 1 _aNordenfelt, Pontus.
_eeditor.
_0(orcid)0000-0002-9481-9951
_1https://orcid.org/0000-0002-9481-9951
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aCollin, Mattias.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071632420
776 0 8 _iPrinted edition:
_z9781071632444
776 0 8 _iPrinted edition:
_z9781071632451
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2674
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-3243-7
912 _aZDB-2-PRO
999 _c5960
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