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020 _a9781071620632
_9978-1-0716-2063-2
024 7 _a10.1007/978-1-0716-2063-2
_2doi
050 4 _aQH460-468
072 7 _aPSAK1
_2bicssc
072 7 _aSCI029000
_2bisacsh
072 7 _aPSAK
_2thema
082 0 4 _a572.86459
_223
245 1 0 _aDNA Damage Responses
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Nima Mosammaparast.
250 _a1st ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aXI, 284 p. 75 illus., 59 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2444
505 0 _aRobust Computational Approaches to Defining Insights on the Interface of DNA Repair with Replication and Transcription in Cancer -- A Whole Genome CRISPR/Cas9 Screening Approach for Identifying Genes Encoding DNA End-Processing Proteins -- Immunoaffinity Purification of Epitope-Tagged DNA Repair Complexes from Human Cells -- Universally Accessible Structural Data on Macromolecular Conformation, Assembly, and Dynamics by Small Angle X-Ray Scattering for DNA Repair Insights -- Assessing DNA damage responses using B lymphocyte cultures -- Studying single stranded DNA gaps a­­t replication intermediates by electron microscopy -- Approaches to monitor termination of DNA replication using Xenopus egg extracts -- Use of high-performance liquid chromatography coupled to mass spectrometry to quantify modified nucleosides -- Targeted Formation of 8-oxoguanine in Telomeres -- Qualitative and Quantitative Analysis of DNA-Cytidine Deaminase Activity -- Characterization of DNA-PK-bound end fragments using GLASS-ChIP -- Monitoring Nuclease Activity by X-ray Scattering Interferometry using Gold Nanoparticle Conjugated DNA -- In Vitro Reconstitution of BRCA1-BARD1/RAD51 Mediated Homologous DNA Pairing -- Purification of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) fromHeLa cells -- Purification and characterization of human DNA ligase III complexes after expression in insect cells -- Generation of monoubiquitin and K63-linked polyubiquitin chains for protein interaction studies.
520 _aThis volume provides detailed methods and key approaches used to mechanistically study DNA damage, as well as the factors involved in the damage response. Chapters guide readers through proteomics and biophysical approaches, analyzing protein function, quantifying DNA replication dynamics and nucleic acid base damage, as well as biochemical reconstitution of key pathways involved in DNA repair. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Damage Responses: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
650 0 _aDNA damage.
650 0 _aDNA repair.
650 1 4 _aDNA Damage and Repair.
700 1 _aMosammaparast, Nima.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071620625
776 0 8 _iPrinted edition:
_z9781071620649
776 0 8 _iPrinted edition:
_z9781071620656
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2444
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2063-2
912 _aZDB-2-PRO
999 _c5936
_d5936