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245 1 0 _aFluorescent Microscopy
_h[electronic resource] /
_cedited by Bryan Heit.
250 _a1st ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aXII, 370 p. 97 illus., 70 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
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347 _atext file
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490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2440
505 0 _a Fluorescence Microscopy: A Field Guide for Biologists -- Three-Dimensional Simultaneous Imaging of Nucleic Acids and Proteins During Influenza Virus Infection in Single Cells Using Confocal Microscopy -- Optimizing Long-Term Live Cell Imaging -- Monitoring Transmembrane and Peripheral Membrane Protein Interactions by Förster Resonance Energy Transfer Using Fluorescence Lifetime Imaging Microscopy -- Bimolecular Fluorescence Complementation to Visualize Protein:Protein Interactions in Cells -- Monitoring Cellular Responses to Infection with Fluorescent Biosensors -- Quantifying Endothelial Transcytosis with Total Internal Reflection Fluorescence Microscopy (TIRF) -- Measurement of Minute Cellular Forces by Traction Force Microscopy -- Quantitative Immunofluorescent Imaging of Immune Cells in Mucosal Tissues -- Intravital Microscopy Techniques to Image Wound Healing in Mouse Skin -- Quantifiable Intravital Light Sheet Microscopy -- Hydrophobic and Hydrogel-based Methods for Passive Tissue Clearing -- Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos -- Super Resolution Radial Fluctuations (SRRF) Microscopy -- A Practical Guide for STED Imaging -- Single-Molecule Localization Microscopy of Subcellular Protein Distribution in Neurons -- Measuring the Lateral Diffusion of Plasma Membrane Receptors using Raster Image Correlation Spectroscopy -- Nanometer-Scale Molecular Mapping by Super-Resolution Fluorescence Microscopy -- Visualizing and Quantifying Data from Timelapse Imaging Experiments -- Automated Segmentation and Microscopy Image Analysis with Machine Learning.
520 _aThis volume provides both experienced and new microscopists with methods and protocols to perform fluorescence microscopy-based experiments. The book is divided into four parts detailing basic fluorescent microscopy, quantitative methods, imaging living animals, human tissue samples, approaches for imaging at a near-molecular level, and approaches to image analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Fluorescent Microscopy aims to be a useful practical guide to researches to help further their study in this field. .
650 0 _aMaterials
_xMicroscopy.
650 1 4 _aMicroscopy.
700 1 _aHeit, Bryan.
_eeditor.
_0(orcid)0000-0003-2392-468X
_1https://orcid.org/0000-0003-2392-468X
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710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071620502
776 0 8 _iPrinted edition:
_z9781071620526
776 0 8 _iPrinted edition:
_z9781071620533
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2440
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2051-9
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