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020 _a9781071618189
_9978-1-0716-1818-9
024 7 _a10.1007/978-1-0716-1818-9
_2doi
050 4 _aQH426-470
072 7 _aPSAK
_2bicssc
072 7 _aSCI029000
_2bisacsh
072 7 _aPSAK
_2thema
082 0 4 _a576.5
_223
100 1 _aPal, Aruna.
_eauthor.
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
245 1 0 _aProtocols in Advanced Genomics and Allied Techniques
_h[electronic resource] /
_cby Aruna Pal.
250 _a1st ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Springer,
_c2022.
300 _aXXIX, 720 p. 254 illus., 176 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aSpringer Protocols Handbooks,
_x1949-2456
505 0 _aChapter 1: Conventional and Basic genomic techniques -- Chapter 2: Recombinant DNA technology -- Chapter 3: Mitochondrial Genetics -- Chapter 4: Basic principle of Cloning -- Chapter 5: Immunohistochemistry -- Chapter 6: Western blotting -- Chapter 7: Introduction to Bioinformatics -- Chapter 8: Molecular evolutionary Study-Phylogenetic tree -- Chapter 9: Real Time or Quantitative PCR -- Chapter 10: Tissue or Cell Cultures -- Chapter 11: DNA Micro Array -- Chapter 12: Epigenetics and DNA Methylation -- Chapter 13: RNA sequencing -- Chapter 14: Next Generation sequencing -- Chapter 15: Metagenomics -- Chapter 16: Genome wide association studies (GWAS)/ SNP chips -- Chapter 17: Exome sequencing -- Chapter 18: RNA Interference -- Chapter 19: Expressing cloned genes for protein production, purification and analysis-Biopharming -- Chapter 20: Transgenesis and Biopharming -- Chapter 21: Rapid amplification of cDNA ends (RACE) -- Chapter 22: Gene Therapy -- Chapter 23: Nutrigenomics -- Chapter 24: Gene Editing -- Chapter 25: Introduction to stem cell biology -- Chapter 26: Transcriptomics -- Chapter 27: Development of Biomarker -- Chapter 28: Single cell genomics -- Chapter 29: Big data Analysis.
520 _aThis laboratory manual includes the latest tools and techniques involved in genomic research. It starts with an introductory chapter on genomics and the various tools and applications involved. The initial chapters present protocols for basic techniques such as DNA isolation, electrophoresis, PCR, cDNA synthesis etc. The book then goes on to describe more advanced techniques such as next-generation sequencing, exome sequencing, use of RNAi, RNAseq, genome editing, single cell genomics etc. Each topic includes a brief description, information on the principles involved, materials & methods, protocol, and expected results, with diagrams and graphs. All protocols are presented in a very lucid and precise way, to make it easy for readers to follow and replicate them.
650 0 _aGenetics.
650 0 _aMedical genetics.
650 0 _aBiotechnology.
650 0 _aBiology
_xTechnique.
650 1 4 _aGenetics and Genomics.
650 2 4 _aMedical Genetics.
650 2 4 _aBiotechnology.
650 2 4 _aBiological Techniques.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071618172
776 0 8 _iPrinted edition:
_z9781071618196
776 0 8 _iPrinted edition:
_z9781071618202
830 0 _aSpringer Protocols Handbooks,
_x1949-2456
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-1818-9
912 _aZDB-2-PRO
999 _c5915
_d5915