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020 _a9781071617991
_9978-1-0716-1799-1
024 7 _a10.1007/978-1-0716-1799-1
_2doi
050 4 _aRB155-155.8
072 7 _aMFN
_2bicssc
072 7 _aMED107000
_2bisacsh
072 7 _aMFN
_2thema
082 0 4 _a616.042
_223
245 1 0 _aPCR Primer Design
_h[electronic resource] /
_cedited by Chhandak Basu.
250 _a3rd ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aXIII, 276 p. 90 illus.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2392
505 0 _aThe significance of PCR primer design in genetic diversity studies; exemplified by recent research into the genetic structure of marine species -- Enhancing cohort PASA efficiency from lessons assimilated by mutant genotyping in C. elegans -- Design of oligonucleotides for allele-specific amplification based on PCR and isothermal techniques -- Detection of rubella virus by tri-primer RT-PCR assay and genotyping by fragment RT-PCR -- Design of mismatch primers to identify and differentiate closely related (sub)species – application to the authentication of meat products -- Primer design for the analysis of closely related species – application of non-coding mtDNA and cpDNA sequences -- Designing PCR primers for the amplification-refractory mutation system -- Validation of circular RNAs by PCR -- Primer Designing for Amplifying an AT-Rich Promoter from Arabidopsis thaliana -- PLASmid TAXonomic PCR (PlasTax-PCR), a multiplex relaxase MOB typing to assort plasmids into taxonomic units -- Multiplex PCR Design for Scalable Resequencing -- Identification of gene copy number in the transgenic plants by quantitative polymerase chain reaction (qPCR) -- qPrimerDB: A powerful and user-friendly database for qPCR primer design -- PCR primer design for the rapidly evolving SARS-CoV-2 genome -- Universal primers for detection of novel plant capsid-less viruses: Papaya umbra-like viruses as example -- A guide to using FASTPCR software for PCR, in silico PCR, and oligonucleotide analysis -- Pyrosequencing Primer Design for Forensic Biology Applications.-Phosphate‌ ‌methylated‌ ‌oligonucleotides‌ ‌as‌ ‌a‌ ‌novel‌ ‌primer‌ ‌for‌ ‌PCR‌ ‌and‌ ‌RT-PCR‌. .
520 _aThis third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR, in silico PCR primer design, and primer design to identify plant and animal viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, PCR Primer Design, Third Edition aims to be useful for various fields of molecular biology, including biotechnology, molecular genetics, and recombinant DNA technology.
650 0 _aMedical genetics.
650 1 4 _aMedical Genetics.
700 1 _aBasu, Chhandak.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071617984
776 0 8 _iPrinted edition:
_z9781071618004
776 0 8 _iPrinted edition:
_z9781071618011
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2392
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-1799-1
912 _aZDB-2-PRO
999 _c5914
_d5914